Ensuring Quality Through Professional Alkynyl-based Cell Surface Glycan Labeling

With state-of-the-art equipment, a highly skilled team of researchers, and a stringent quality control system, CD BioGlyco excels in delivering rapid, precise, and top-notch GlycoCLICK™-based Labeling Service. Our alkynyl-based GlycoCLICK™-based Cell Surface Glycan Labeling Service empowers clients to achieve greater efficiency and accuracy in quantifying and localizing cell surface glycans for their research endeavors. In addition to an extensive range of alkynyl-based cell surface glycan labeling services, CD BioGlyco offers an array of other specialized solutions to cater to diverse research needs.

• Alkynyl fucose-based cell surface glycan labeling service
  We use alkynyl fucose to interact with intracellular substrates to generate alkynyl glycan molecules. The alkynyl fucose is then added to the medium in which the cells are cultured. These alkynyl glycan molecules can bind to probes containing azide functional groups via click chemistry to achieve cell surface glycan labeling. We select alkynyl fucose and azide and perform probe design according to your research needs to ensure reaction specificity and efficiency. In addition, we also evaluate and optimize aspects such as cytotoxicity and cell permeability to ensure the reliability and feasibility of the method.
• Alkynyl N-azidoacetylmannosamine (ManNAz)-based cell surface glycan labeling service
  First, we introduce the alkyne group into the ManNAz molecule by chemical synthesis or enzyme catalysis. The alkyne-based ManNAz is then added to the medium in which the cells are cultured and incubated. Subsequently, a probe containing an azide functional group is added to the culture medium and allowed to react with the alkynyl ManNAz on the cell surface in a click reaction to achieve labeling. Finally, the labeled cells are visualized or detected by fluorescent dyes or other detection methods to obtain information about the cell surface glycan groups.

Fig.1 Process of alkynyl-based cell surface glycan labeling. (CD BioGlyco)

Publication Data

Applications

• Specific labeling of cell surface glycans by the click reaction is used for the analysis of cell surface glycans.
• By co-treating cells with drug molecules and alkylating agents, the technique can be used to combine drugs and cell surface glycans via a click reaction, thus evaluating drug efficacy and selectivity.
• By introducing glycan synthesis precursors or substrates containing alkyne groups into the cell, researchers use click chemistry to convert them into specific glycan structures that can be used for glycan engineering or synthetic biology research.

Advantages

• It targets specific sugar molecules for labeling without interfering with the function and structure of other cellular components.
• The reaction is usually completed in a relatively short time and the labeling signal can be observed within a few hours.
• This is generally done under mild conditions that help maintain the integrity and life of the cells.

CD BioGlyco is committed to providing clients with personalized alkynyl-based cell surface glycan labeling services. We have a deep understanding of our clients' needs and customize our solutions according to different experimental purposes and sample characteristics. Please feel free to contact us and we will be happy to assist you in your research.

• Azide-based Cell Surface Glycan Labeling Service