Unlock the Power of Glycoscience with GlycoCLICK™-based Chemical Lysis

The primary objective of introducing molecules into a biological sample before specific lysis at designated sites within the sample is to achieve precise localization and subsequent release of targeted biological molecules or structures. This technique is frequently employed when isolating, enriching, or analyzing specific components becomes necessary, thereby enhancing the sensitivity and accuracy of target molecule detection. CD BioGlyco has been offering professional GlycoCLICK™-based Decoupling Service to our clients for numerous years, encompassing the GlycoCLICK™-based Enzymatic Lysis Service, the GlycoCLICK™-based Photolysis Service, and the tailored GlycoCLICK™-based chemical lysis service.

• We use different markers such as biotin, fluorescent markers, etc. to achieve the lysis of biological samples. The selected functional groups, like biotin and fluorescent markers, are introduced into the biological samples by chemical cross-linking or bio-covalent binding, and then the lysis method is selected on a case-by-case basis. In this way, the biomolecules or cellular structures inside the sample are disrupted, releasing the target molecules or other components to be analyzed, thus enabling the localization and release of specific biomolecules or structures. For instance, if you need to detect the expression of a certain protein in a biological sample, we select an antibody with affinity as a functional group, introduce it into the sample, and bind it to the target protein. The protein is then released by lysis and then detected and quantified by western blotting.
• We offer different targeted lysis services depending on the type or property of the target molecule to be released, of which common classifications include protein lysis, nucleic acid lysis, small molecule compound lysis, and cell lysis. By targeting specific types of target molecules for cleavage, we optimize the cleavage strategy and improve the selectivity and efficiency of cleavage, thus better enabling the processing and analysis of target molecules.

Fig.1 GlycoCLICK™-based chemical lysis services. (CD BioGlyco)

Publication Data

Applications

• In biomarker research and applications, chemical decoupling can be applied to remove click-chemically introduced markers from a molecule so that the nature and activity of the target molecule can be accurately determined.
• It can be applied to the modification and functionalization of compounds, such as the removal of unwanted functional groups or markers, or the introduction of new functional groups to change the properties of the compound.
• In analytical experiments, chemical lysis is used to reduce or eliminate interfering substances introduced by click chemistry, improving the accuracy and reliability of the analysis.

Advantages

• GlycoCLICK™-based chemical lysis is highly customizable, allowing the introduction of different functional groups for the lysis of different types of biological samples according to specific needs.
• GlycoCLICK™-based chemical lysis is highly selective and reacts with the target biomolecule via introducing specific functional groups to enhance specificity and accuracy in the process.
• We provide clear and reliable results and reports to ensure that clients have an accurate understanding of the process and results.

CD BioGlyco provides GlycoCLICK™-based chemical lysis services to achieve efficient lysis of different types of biological samples by customizing chemical lysis protocols to release target molecules for subsequent analysis. If you need any further information, please feel free to contact us.

• GlycoCLICK™-based Enzymatic Lysis Service
• GlycoCLICK™-based Photolysis Service