GlycoCLICK™-based Glycoprotein Synthesis Service
Rigorous and Attentive Glycoprotein Synthesis Service
CD BioGlyco has successfully synthesized a variety of glycoconjugates such as Glycopolymer, Glycopeptide, and glycoprotein using Click Chemistry. Glycoproteins have a large number of polysaccharide side chains and a wide range of biological functions. In order to meet the research needs of our clients regarding the biological activities of glycoproteins, we have developed a one-stop GlycoCLICK™-based glycoprotein synthesis service. Our strategy for glycoprotein synthesis is mainly based on mimics of protein backbones. The additional functions of amino acid sequences are utilized to control the position of glycosidic moieties, and finally, glycoproteins with good biophysical properties are successfully synthesized.
After determining the structure and expected physicochemical properties of the glycoproteins, we first synthesize the sugars and proteins separately. After that, we perform chemical glycosylation of the protein backbone. Their chemical glycosylation involves the creation of synthetic bonds between proteins and sugars. We use chemical functional groups not used in natural glycosylation such as thiols, azides, phenols, and alkynes. Among them, azides and alkynes are utilized copper(I)-catalyzed alkyne-azide cycloadditions (CuAACs), which are reacted with a good level of conversion. Glycoproteins are finally synthesized successfully by ligation.
During the synthesis, we consider the presence of protecting groups and the effect of sugar chain length on the physical properties and reaction efficiency of glycoproteins. Also, all compounds in the synthesis are characterized by sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE), matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), and nuclear magnetic resonance (NMR).
Publication Data
Technology: CuAAC
Journal: Bioconjugate Chemistry
IF: 4.774
Published: 2020
Results: The present study provides a new route to glycan-mimetic proteins based on the combination of a CuAAC driver peptide glycosylation platform and global amino acid substitutions. The effects of global amino acid substitutions on the physical properties and expression of the proteins were investigated during the synthesis. The efficiency of CuAAC coupling was also investigated by protected and deprotected monosaccharides and so on. The results showed that the glycoprotein synthesized by this strategy is a functionalized mimic with good properties. This study provides an efficient strategy for glycoprotein synthesis.
Fig.1 A description of the protein design and synthetic method. (Seifried, et al., 2020)
Applications
- GlycoCLICK™-based glycoprotein synthesis plays an important role in studying the effect of glycan chains on glycoprotein solubility, folding, overall conformation, etc.
- GlycoCLICK™-based glycoprotein synthesis plays an important role in studying and regulating the biological functions of glycoproteins, including cellular recognition, structural support, and so on.
- Many pathogens utilize host cell surface glycoproteins for recognition and binding. GlycoCLICK™-based glycoprotein synthesis has been used to study pathogen recognition mechanisms.
Highlights of Us
- We have hundreds of high-quality click chemistry-related series products for glycoprotein synthesis.
- We are equipped with first-class precision instruments for glycoprotein synthesis, purification, quality testing, and analysis.
- We ensure 100% client satisfaction and successful completion of projects from product consultation, R&D, synthesis, and packaging to after-sales tracking and technical support.
CD BioGlyco continues to carry out synthesis research and development of glycoproteins and others through click chemistry. Please feel free to contact us to learn more about glycoprotein synthesis and tell us the purpose of your experiment. With our philosophy of prioritizing quality and service, we will excel in fulfilling any of your needs.
Reference
- Seifried, B.M.; et al. Glycoprotein mimics with tunable functionalization through global amino acid substitution and copper click chemistry. Bioconjugate Chemistry. 2020, 31(3): 554-566.
For research use only. Not intended for any clinical use.
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