Reliable Assistant for Oligosaccharide Scaffold Materials Preparation

During the reconstruction of tissues and organs, the three-dimensional structure used to seed cell regeneration and on which the tissue depends on survival and attachment is called a scaffold. They are mainly made of biodegradable and absorbable biomaterials, called biomaterial scaffolds. At CD BioGlyco, we have developed specialized one-stop solutions for Biomaterial Preparation to help clients design biomaterials, synthesize biomaterials, characterize biomaterials, and evaluate the properties of biomaterials. Many oligosaccharides have good potential in the field of biomaterials. We develop oligosaccharide-based bioscaffold materials to assist client's research in the fields of bone tissue engineering, neural tissue engineering, vascular tissue engineering, corneal tissue engineering, and other fields. We provide clients with the preparation services of the following oligosaccharide scaffold materials:

• Hydrogel preparation service
• Fiber scaffold preparation service
• Polymer film preparation service
• Other oligosaccharide scaffold preparation service

Click chemistry reactions provide a versatile method for preparing bioactive scaffold materials with complex chemical compositions and physical properties. This method requires less work-up, shorter reaction times, minimal setup, and higher yields. In the preparation of oligosaccharide scaffold materials, the click chemistry technologies we use include but are not limited to:

• Copper(I)-catalyzed alkyne-azide cycloaddition (CuAAC): CuAAC is one of the commonly used click reactions. It is a reaction between a functionalized azide molecule and a functionalized terminal alkyne molecule to form a thermally and hydrolytically stable triazole ring. Due to the advantages in versatility and reaction efficiency, we conjugate functional biomolecules to the scaffold body through the CuAAC reaction to construct bioactive scaffold materials to assist clients in their biomedical research.
• Strain-promoted alkyne-azide cycloaddition (SPAAC): The SPAAC reaction is efficient, fast, highly selective, and bio-orthogonal, which makes it have good application prospects in the field of materials science. We utilize the excellent properties of the SPAAC reaction to prepare films, coatings, adhesives, functional polymers, and dendrimers, or for surface modification of scaffold materials.
• Diels-Alder reaction: We perform highly selective [4+2] cycloaddition of dienophiles and dienophiles to construct stable cyclohexene adduct products and prepare functionalized biomaterials.

Publication

Applications

• Bone tissue scaffold development: Many oligosaccharides have good biocompatibility and mechanical stress, and have unique advantages in the research of bone tissue scaffolds. They not only simulate the living environment of osteoblasts but also recreate the mechanical stress of the tissue.
• Nerve tissue scaffold development: Nerve scaffold materials act as a bridge between two damaged nerve terminals and provide corresponding support for the healing production between the two parts of the tissue. We help clients develop chitosan-based neural tissue scaffold materials.
• Skin tissue scaffold development: Coating oligosaccharides on the surface of scaffold materials is used to repair damaged skin tissue.

Frequently Asked Questions

• What are the advantages of click reactions in scaffold material preparation?

Click chemistry is to connect structural skeletons and building blocks through efficient, reliable, and regionally selective chemical reactions. The core of click chemistry is to use a series of efficient, reliable, and selective C—X bonding reactions to achieve the modular preparation of a large number of new compounds. Click reactions usually have the following characteristics: (1) The raw materials used are easily available; (2) The reaction operation is simple, the conditions are mild, and it is not sensitive to oxygen and water; (3) The product yield is high and the stereoselectivity is good; (4) It is fast and high-throughput modular synthesis; (5) The product is easy to purify, post-reaction treatment and product separation are simple and convenient, and the by-products are environmentally friendly.

• Why are click chemistry reactions uniquely attractive in biomedicine?

First, the azide group and the alkyne group hardly react with biomolecules, and their polarity is relatively weak. They have no obvious effect on the properties of other groups connected to them and are easily introduced into compounds; Secondly, the click reaction is carried out in an aqueous medium, so the reaction is carried out under physiological conditions; More importantly, click reactions have very high chemical selectivity and are used to modify highly functional biomolecules, such as peptides, nucleic acids, polysaccharides, etc.

CD BioGlyco uses click chemistry reactions with simple operation, mild conditions, and high reaction yields to help clients prepare and modify oligosaccharide scaffold materials. If you have any needs, please feel free to contact us.

• GlycoCLICK™-based Nanoparticle Preparation Service
• GlycoCLICK™-based Fluorescent Probe Preparation Service
• GlycoCLICK™-based Biosensor Preparation Service
• GlycoCLICK™-based Liposome Preparation Service
• GlycoCLICK™-based Hybrid Sugar Material Preparation Service
• GlycoCLICK™-based Self-assembled Monolayer (SAM) Scaffold Preparation Service
• GlycoCLICK™-based Polyamidoamine Polycationic Scaffold Preparation Service
• GlycoCLICK™-based Multivalent Click Sugar Carbon Nanotube (CNT) Preparation Service
• GlycoCLICK™-based β-cyclodextrin (CD) Biomaterial Preparation Service