Powering Precision with Carbohydrate Click for Next-Gen Galectin Inhibitors

Galectins act through a "receptor"-"ligand" mechanism during apoptosis and metastasis of tumor cells. The use of carbohydrates to develop relevant inhibitors effectively interferes with the galectin and ligand binding pathway. At CD BioGlyco, we provide systematic Lectin Ligand Development Services that are effectively integrated with Drug Development Services. Here, we provide our clients with high-quality carbohydrate click-based galectin inhibitor development services.

We utilize Cu(I)-assisted cycloaddition reactions of alkynes and azides to generate linear oligogalactopyranosides and use them to construct libraries of galectin inhibitors. The conditions throughout the reaction are mild and efficient, no protecting groups are required, and purification is not necessary in most cases.

• In the first stage of library synthesis, we utilize click chemistry to generate functionalized ketocarboxylic acids containing different alkynes. Then, the compounds in the library are tested for preliminary inhibitory activity, and the binding sites of the compounds on the carbohydrate recognition domain of galectin are analyzed, and compounds with high inhibitory activity against galectin are screened in a high-throughput manner.
• In the second stage, we continued to utilize click chemistry to bind the compounds screened in the previous step to the alkyne library assembly. Then, large libraries of efficient galectin inhibitors are constructed. Due to the efficiency of the click reaction and the water compatibility, in most cases, the assembled products can be screened directly as inhibitors without any purification.

Throughout the process, we provide animal models or cellular models for in vitro experiments to analyze the inhibitory activity and semi-inhibitory concentration values of galectin inhibitors in the library. We also provide fluorescent labeling methods to analyze the binding sites of compounds in the inhibitor library to the carbohydrate recognition domain (CRD) of galectin.

Fig.1 Process of developing galectin inhibitors based on carbohydrate clicks. (CD BioGlyco)

Publication Data

Applications

• Galectins are involved in the regulation of cell growth and differentiation and are closely related to the whole process of tumor formation, development, and metastasis. Therefore, galectin inhibitor development can be used for further development of antitumor and anticancer drugs.
• Carbohydrate click-based development of galectin inhibitors can be used to explore the function of galectin in signaling, and other biological processes produced.

Advantages

• We offer inhibitor development technology for a wide range of applications with high yields and strong stereoselectivity.
• We offer large-scale production of inhibitor products and our products are highly stable.
• We have a professional inhibitor development team and provide custom inhibitor development solutions.

CD BioGlyco has extensive experience in click chemistry research. With our first-class click chemistry technology, we provide our clients with high-quality drug development services. For example, inhibitor development, antagonist development, and so on. If you are interested in our click chemistry services, please feel free to contact us.

• Carbohydrate Click-based Selectin Antagonist Development Service