GlycoCLICK™-based Fluorescent Probe Preparation Service
Developing Market-competitive Fluorescent Probes Is Always Our Goal
Fluorescent labeling technology combines fluorescent substances with the components to be measured through physical or chemical effects so that the components to be measured and the fluorescent substances combine to form a complex. Qualitative or quantitative information about the components to be measured is obtained through fluorescence detection. With the development of modern medicine and molecular biology and the application of various advanced fluorescence detection technologies and instruments, such as flow cytometer (FCM), laser scanning confocal microscope (LSCM), and other instruments, fluorescent labeling, as a non-radioactive labeling technology, has received widespread attention and has developed rapidly. CD BioGlyco is a world-class expert in glycobiology, providing high-level, competitive fluorescent probe preparation services to clients from all over the world through GlycoCLICK™ Chemistry technology. In addition, we also provide other Biological Material Preparation services to global clients, including but not limited to:
Our GlycoCLICK™-based fluorescent probe preparation services are as follows:
- Fluorescent probe molecule design services
In order to design and develop fluorescent probes with good performance, we focus on the relationship between dye performance and molecular structure. The dye's fluorescence properties, photostability, solubility properties, low pH sensitivity in solution, the possibility of preparing different types of active derivatives, intrinsic low toxicity, and biological activity are important factors that we consider.
- Fluorescent probe molecule synthesis services
We use click chemistry technology to continuously optimize synthesis methods, shorten synthesis routes, improve reaction yields, and explore effective separation methods to obtain high-purity fluorescent probe molecules.
- Fluorescent probe molecule modification services
Molecular modification of polysaccharides changes their spatial structure, relative molecular mass, and substituent types, numbers, and positions, thereby affecting the biological activity of polysaccharides. Choose appropriate methods to molecularly modify polysaccharides to obtain different derivatives and improve their biological activity. Therefore, molecular modification of polysaccharides has become an important means to develop polysaccharide fluorescent probes.
Publication
Technology: In situ click chemistry
Journal: Scientific Reports
IF: 4.6
Published: 2015
Results: Target-guided synthesis is a drug discovery approach that allows targets to self-assemble their own binders. In situ click chemistry is a target-guided synthesis technique through which many drugs that bind nucleic acids and proteins with strong affinity have been discovered. Here, the authors adopted an in situ click chemistry approach to developing a parallel G-quadruplex fluorescent probe based on a triarylimidazole scaffold with good selectivity and sensitivity.
Fig.1 In situ click chemistry for developing a parallel G-quadruplex fluorescent probe. (Hu, et al., 2015)
Applications
- Fluorescent dyes immobilized on bioactive carriers utilize the selectivity between bioactive substances to achieve the purpose of selectively identifying and locating mutant cells. For example, combining dyes with antibodies or certain protein carriers for tumor detection can be used in many anti-cancer drugs.
- Certain fluorescent dyes identify certain types of cells, selectively enter and label them, and are directly used to identify mutant cells. For example, some cyanine dyes are used directly to label mutated cells or as radiosensitizers in the treatment of solid tumors.
- Fluorescent probes are used for the determination of environmental pollutants, drugs, amino acids, chiral molecules, nucleotides, and other substances. They are of great significance in many life chemical processes such as enzymatic reactions, immune reactions, and protein biosynthesis.
Frequently Asked Questions
- What are the commonly used fluorescent dyes for biomarkers?
According to the way fluorescent dye probe molecules label cells, fluorescent dyes are divided into fluorescent probes containing active groups and embedded fluorescent probes. Fluorescent probes containing active groups bond with labeled substances, such as rhodamines, fluoresceins, naphthalimides, stilbenes, acridines, fluorenes, etc. The mode of interaction between embedded fluorescent probes and DNA is to insert into the structure of DNA through affinity, such as thiazole orange (TO) and oxazole yellow (YO) series of cyanine dyes. Cyanine dyes do not emit light when not combined with nucleic acids, and there is no background interference during detection, which is lacking in previous dyes. When combined with DNA, especially when combined with ds-DNA, its fluorescence intensity increases by more than 1000 times. When combined with RNA, its fluorescence intensity increases to more than 3000 times. This dye has a higher affinity for tumor cells than normal cells and is widely used in the early labeling of cancer cells.
- What are the advantages of using fluorescent dyes for labeled analysis?
Fluorescent dye is a widely used fluorescent labeling agent (also known as fluorescent probe), which has the advantages of fast detection speed, good repeatability, small sample volume, and no radiation. Fluorescent probes are used to determine the structure of RNA and DNA, study DNA base damage repair, identify the status of amino groups in protein molecules and the active areas of protein molecules, detect pmol-level proteins, distinguish nucleic acids with different conformations, and chemical reactions active related to drugs.
CD BioGlyco has accumulated rich experience in the development of biological materials. We help you design and develop high-stability, high-sensitivity, and high-selectivity biological materials based on your needs to assist your research. If you happen to have a biomaterials development project in hand, please contact us in time and we will develop an exclusive customized plan to meet your needs.
Reference
- Hu, M.H.; et al. A new application of click chemistry in situ: development of fluorescent probe for specific G-quadruplex topology. Scientific Reports. 2015, 5(1): 17202.
For research use only. Not intended for any clinical use.
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