GlycoCLICK™-based Decoupling Service

GlycoCLICK™-based Decoupling Service at CD BioGlyco

At CD BioGlyco, we have a dedicated team of experts in the field of glycobiology with over 10 years of experience to support you with our GlycoCLICK™-based decoupling service. CD BioGlyco provides a comprehensive solution for your research project, ensuring the success of your projects in various aspects. Our glycochemistry and glycobiology experts are on hand to advise on every stage of the project.

GlycoCLICK™-based Enzymatic Lysis Service

CD BioGlyco is experienced in providing GlycoCLICK™-based enzymatic lysis services that are designed to facilitate the lysis and extraction of target molecules from cell or tissue samples. Our advanced approach enables the rapid and efficient breakdown of cells or tissues, allowing for the extraction of specific molecules that can be further analyzed and applied in various research and practical applications.

GlycoCLICK™-based Photolysis Service

CD BioGlyco offers expert GlycoCLICK™-based photolysis services that employ targeted photosensitizers and light of particular wavelengths to activate intracellular photosensitizers. This leads to a photochemical reaction that causes the breakdown of the cell membrane and facilitates the release of intracellular components.

GlycoCLICK™-based Chemical Lysis Service

CD BioGlyco provides our clients with a specialized GlycoCLICK™-based chemical lysis service using state-of-the-art technology. The chemical lysis method utilizes chemical reagents such as detergents, lysis buffers, and enzymes to dissolve lipids and proteins in cell membranes, creating pores and lysing cells.

Techniques typically used in our GlycoCLICK™-based decoupling include but are not limited to:

Biomolecular labeling

To perform the click reaction on cells, specific biomolecular labels, such as sugars or proteins, need to be introduced on the cell surface. These labels present specificity on the cell surface and can bind to the functional groups of the click reaction.

Spectral analysis

After performing cell lysis, spectral analysis of the lysed biomolecules, such as mass spectrometry and fluorescence spectrometry, is required to determine the lysis effect and to obtain relevant structural and functional information.

Fig.1 Classification of the decoupling method. (CD BioGlyco)

Publication Data

Technology: Cell lysis, microfluidic, and fluorescence staining

Journal: Small

IF: 15.153

Published: 2019

Results: This article focuses on a piezoelectric microchip for cell lysis based on cell-particle collisions in microdroplets driven by surface acoustic wave oscillations. The researchers used a piezoelectric microchip for cell lysis by colliding cells with particles through the movement of microdroplets generated by acoustic surface wave oscillations. The optimal operating conditions for efficient cell lysis were determined by investigating the effects of wave frequency as well as the density and size of the particles on cell lysis efficiency. In addition, the researchers used fluorescence microscopy and staining techniques to observe and analyze the cells. This rapid and efficient cell lysis method with a simple manufacturing process, low cost, controlled lysis, and physiological maintenance of the biological components released by the cells will be widely used in future on-site cellular bioassays, single-cell sequencing in cell biology research, disease diagnostics, and stem cell therapy research.

Fig.2 Schematic process of cell lysis. (Wang,et al., 2019)

Applications

GlycoCLICK™-based decoupling can be used not only for the extraction of intracellular substances but also for the detection of pathogens, drug screening, evaluation, which has a wide range of application prospects and potentials.
Cell lysis releases intracellular proteins by disrupting cell membranes, which is applied for protein isolation, purification, and analysis.
Cell lysis is used to extract intracellular DNA or RNA for subsequent genomics or transcriptomics studies.
Cell lysis releases various intracellular components, which facilitates quantitative analysis and functional studies of intracellular metabolites, signaling molecules, and enzyme activities.

Advantages

We customize different cell lysis solutions and services according to the needs of clients, such as adjusting lysis conditions to meet your different applications.
Our technicians have extensive experience and expertise in cell lysis technology to provide you with high-quality and reliable services.

CD BioGlyco offers a comprehensive range of GlycoCLICK™-based decoupling services to support our clients in their research endeavors. Whether you are initiating new research or require assistance with an ongoing project, we are dedicated to assisting you every step of the way. If you need the related services, please feel free to contact us for more detailed information.

Reference

Wang, S.;et al. Cell lysis: piezoelectric microchip for cell lysis through cell-microparticle collision within a microdroplet driven by surface acoustic wave oscillation. Small. 2019, 15(9): 1970050.

For research use only. Not intended for any clinical use.

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