GlycoCLICK™-based Glycopeptide Synthesis Service
Advanced Glycopeptide Services: Custom Synthesis and Analysis Solutions at CD BioGlyco
Sugar molecules play important roles in many biological processes such as cell signaling, inflammatory responses, and pathogen recognition. CD BioGlyco uses Click Chemistry Technology to combine sugar groups with peptide segments to synthesize glycopeptide complexes with modulated biological activity to support clients' different research needs.
- Glycopeptide synthesis service
Glycopeptides have the characteristics of both sugar and peptide modules and interact with both sugar receptors and peptide receptors, which makes them ideal tools for designing highly selective and specific recognition ligands for the study of glycoproteins, and other biomolecules. Based on different click chemistry technologies, CD BioGlyco provides mature glycopeptide synthesis services by selecting appropriate reaction conditions and click chemistry methods. We have the technology and facilities to synthesize complex glycopeptide molecules and design and synthesize specific glycopeptide structures according to client needs.
- Cu(I)-catalyzed coupling reaction: This is a commonly used click chemistry method in which we synthesize glycopeptides by reacting amino-containing peptides with alkenyl-functional sugars. The reaction uses copper ions as a catalyst to quickly form a stable ring product.
- Azide-alkyne cycloaddition reaction: We use click chemistry to perform a cycloaddition reaction between an alkynyl group and an azo compound (azide) to synthesize glycopeptides.
- Thiol-ene coupling (TEC): We choose compounds containing alkenyl and thiol functional groups as starting materials to synthesize glycopeptides. Then we perform the TEC reaction under neutral or slightly acidic conditions and promote the reaction by adding photoinitiators or thermal initiators.
- Glycopeptide modification service
We use click chemistry technology to provide glycopeptide modification services. By using click reactions to introduce different functional groups, such as fluorescent dyes, tags, or other bioactive molecules, to give glycopeptides new properties and functions.
- Glycopeptide screening and analysis service
We utilize click chemistry technologies to develop tools and methods for glycopeptide screening and analysis. For example, we utilize click reaction design to develop fluorescent probes to detect specific glycopeptide structures; We used click chemistry Labeling Technology to label glycopeptides for quantitative analysis. Moreover, glycopeptides are used as components of targeted Drug Delivery systems, we combine glycopeptides with drugs or nanoparticles through click reactions to achieve efficient drug delivery targeting specific cells or tissues.
Fig.1 One-stop glycopeptide synthesis and analysis. (CD BioGlyco)
Publication
Technology: TEC reaction
Journal: Tetrahedron Letters
IF: 2.032
Published: 2011
Results: The authors prepared S-glucosyl glutathione through the light-induced TEC reaction and separated the target S-glycopeptide through chromatography, with a yield of 97%. The authors demonstrated that this clicking process was performed under very mild conditions and required a small amount of energy, which opened the way for future applications, especially in bioconjugation.
Fig.2 Synthetic route of S-glycopeptide. (Fiore, et al., 2011)
Applications
- Vaccine R&D: The use of GlycoCLICK™ technology to synthesize glycopeptides mimics the structure of pathogen surface glycoproteins, it is used for vaccine development and immunotherapy to provide efficient immune responses against specific pathogens.
- Cancer research: GlycoCLICK™-based glycopeptide synthesis is used to synthesize cancer-related glycopeptides, helping researchers understand specific glycosyl modifications on tumor cells and explore their role in cancer development.
- Drug screening and development: GlycoCLICK™-based glycopeptide synthesis is used to synthesize drug candidates targeting specific disease targets and provide support for the development of new drugs.
Advantages
- Based on clients' requirements and research purposes, we provide custom synthesis services, including specific glycosyl modifications, glycopeptides of different lengths and sequences, etc., to meet clients' individual needs.
- GlycoCLICK™ technology has the characteristics of high reaction selectivity and high yield. We use this technology to synthesize glycopeptides more efficiently, reduce synthesis steps and material consumption, and improve output efficiency.
- We are equipped with advanced synthesis equipment and laboratory facilities, including high-performance liquid chromatography (HPLC), mass spectrometer, etc., to provide strong support for the monitoring and analysis of the synthesis process.
CD BioGlyco has a professional scientist and R&D team with rich synthesis experience and expertise. We provide high-level GlycoCLICK™-based glycopeptide synthesis services. Please feel free to contact us if you would like to obtain detailed service details.
Reference
- Fiore, M.; et al. Synthesis of S-glycosyl amino acids and S-glycopeptides via photoinduced click thiol-ene coupling. Tetrahedron Letters. 2011, 52(3): 444-447.
For research use only. Not intended for any clinical use.
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