GlycoCLICK™-based Oxime Skeleton Molecule Imaging Service

GlycoCLICK™-based Oxime Skeleton Molecule Imaging Service

Your Trusted Oxime Skeleton Molecule Imaging Partner

CD BioGlyco has built on our expertise over the years to provide best-in-class GlycoCLICK™-based Imaging Service to our clients around the world. Among them, our clients have shown great appreciation for GlycoCLICK™-based Disulfide Bonded Molecule Imaging Service and oxime skeleton molecule imaging services. The details of our GlycoCLICK™-based oxime skeleton molecule imaging service are as follows.

  • CD BioGlyco designs fluorescent probes containing oxime groups according to your experimental needs to realize the imaging of specific molecules or bioactive substances in organisms. This method enables visual observation of target molecules through the detection and localization of fluorescent signals and is commonly used in the fields of cell imaging, biomarkers, and drug release.
  • We design biosensors based on oxime molecular structures to help you realize the detection and imaging of biomolecular concentration, activity, and other biological information. This approach often enables real-time monitoring of dynamic changes in biological systems.
  • Our experts utilize the property of oxime skeleton to form stable compounds with metal nanoparticles, combined with surface-enhanced Raman scattering technology to achieve highly sensitive imaging of oxime molecules. This can be used for applications such as surface analysis and interface detection.
  • We achieve multimodal imaging by combining oxime backbone molecules with other imaging techniques to provide you with more comprehensive information and more accurate imaging results. This approach allows for a multifaceted view of the target at different levels and angles, improving imaging accuracy and visualization.

Fig.1 Classification of oxime skeleton molecule imaging by utilizing GlycoCLICK™ technology. Fig.1 GlycoCLICK™-based oxime skeleton molecule imaging service. (CD BioGlyco)

Publication Data

Technology: Photochemical reaction

Journal: Chemical Science

IF: 8.4

Published: 2021

Results: This article describes a novel visible-light photoactivated fluorescent substance that uses an oxime group as a photodissociative moiety. The researchers caused the fluorescent substance to lose its fluorescent properties by introducing the oxime group into it. Under conditions of light irradiation and the presence of air, the oxime group was oxidized to its ketone derivative, which restored the strong fluorescence. The researchers also used this oxime moiety to design probes targeting different organelles for confocal imaging of living cells. In addition, they performed photoactivated localization microscopy (PALM) imaging using low-power photoactivation under physiological conditions without the use of cytotoxic additives. The results suggest that the oxime moiety is a novel visible light photodissociation moiety that can be widely used in cellular imaging, sensing, and light-controlled molecular release.

Fig.2 Summary of the design of oxime-caged fluorogenic dyes. Fig.2 Overview of the oxime-caged fluorogenic dyes design in this study. (Wang, et al., 2021)

Applications

  • GlycoCLICK™-based oxime skeleton molecule imaging can be used for real-time confocal imaging of different organelles within a cell, as well as for PALM imaging using low-power photoactivation under physiological conditions.
  • This fluorescence imaging technique can be combined with genetically encoded labeling techniques for super-resolution imaging of proteins of interest.
  • By combining fluorescently labeled oxime skeleton-based molecules with drug carriers, real-time monitoring and imaging of drug delivery and release processes in vivo can be achieved, providing important information for drug effect assessment.

Advantages

  • Oxime skeleton-based fluorescent probes can be combined with other imaging techniques to realize multimodal imaging.
  • Oxime molecules possess versatile and adaptable chemical structures that can be strategically tailored and refined through synthetic chemistry to cater to diverse applications.
  • Oxime skeleton molecules can be strategically designed and chemically tailored to finely tune their optical characteristics, thereby enabling seamless adaptation to diverse fluorescence imaging instruments and experimental requirements.

CD BioGlyco is proud to offer a cutting-edge and highly specialized GlycoCLICK™-based oxime skeleton molecule imaging service. With our advanced GlycoCLICK™ technology, we provide tailored molecular imaging solutions that cater specifically to your research needs. If you need further details about our service, please feel free to contact us.

Reference

  1. Wang, L.; et al. Oxime as a general photocage for the design of visible light photo-activatable fluorophores. Chemical Science. 2021, 12(47): 15572-15580.
For research use only. Not intended for any clinical use.
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