GlycoCLICK™-based Drug Development Service
GlycoCLICK™-based Drug Development Service at CD BioGlyco
Click chemistry reactions are an important part of the medicinal chemistry toolbox, which is used in constructing compound libraries and helps to discover bioactive compounds and drugs. CD BioGlyco has extensive experience in the field of synthesis, and we provide efficient GlycoCLICK™-based drug development service.
Click reaction-based design service
We design click chemistry reaction protocols suitable for drug molecules according to specific needs. Copper-catalyzed azide-alkyne cycloaddition (CuAAC) click chemistry is a bioorthogonal reaction widely used in medicinal chemistry.
Click reagent synthesis service
We synthesize the corresponding click reagents through chemical synthesis, enzymatic reaction, or biosynthesis. These reagents often include click-active groups and adapter sites, such as the synthesis of compounds with alkyne and azide groups.
Click reaction-based compound library construction service
We perform click reactions with click reagents and different substrate structures to achieve the synthesis of diverse compounds. The substrate is a compound in an existing compound library or a new compound designed and synthesized according to needs. Click-based reactions facilitate the rapid construction of compound libraries.
Compound screening service
We use high-throughput screening technology to screen compound libraries and develop target compounds based on different targets and modes of action (such as targeting enzymes or lectins). This process helps to accelerate the drug development process.
We use enzymes as targets and use click technology to glycosylate enzymes, which changes the activity, stability, and specificity of enzymes, thereby developing selective and efficient enzyme inhibitors, For example, Protein Tyrosine Phosphatase (PTP) Inhibitor, Protein Kinase Inhibitor, Fucosyltransferase Inhibitor, Mannosyltransferase Inhibitor, Nicotinamide Phosphoribosyltransferase Inhibitor, O-GlcNAcase Inhibitor, Glycogen Phosphorylase Inhibitor, Glycosidase Inhibitor, and Neuraminidase Inhibitor. These inhibitors are widely applied in the fields of enzyme biological functions and drug development.
We use reliable carbohydrate click reactions to synthesize and modify carbohydrate compounds for the development and research of lectin ligands, for example, developing Galectin Inhibitor and Selectin Antagonist. The development of lectin ligands is of great significance for biological research.
Compound synthesis and identification service
We provide professional synthesis and purification for the compounds obtained through screening. Common purification methods include column chromatography, solvent extraction, and gel electrophoresis. At the same time, we conduct comprehensive structural characterization and analysis of the purified target compound to ensure its quality and purity, which may involve nuclear magnetic resonance, mass spectrometry, high-performance liquid chromatography (HPLC), and other technologies.
Compound biological evaluation service
We evaluate the activity and pharmacokinetic properties of target compounds through appropriate biological experiments or in vitro tests, which help determine the pharmacological properties and potential pharmaceutical effects of the compound. In addition, we also provide mature GlycoCLICK™-based Drug Delivery Service.
Fig.1 GlycoCLICK™-based drug development service. (CD BioGlyco)
Publication
Technology: In situ click chemistry
Journal: European Journal of Medicinal Chemistry
IF: 7.088
Published: 2020
Results: The authors used HIV-1 RT as a target, synthesized a small combinatorial library using CuAAC reaction of click chemistry, and identified 22 effective HIV-1 NNRTIs through in situ screening. These compounds were then further synthesized and evaluated for their anti-HIV-1 activity in vitro. The results showed that A3N19 had the strongest activity against HIV-1 IIIB. The authors believed that the strategy of in situ click chemistry-based was suitable for the future discovery of more effective HIV-1 NNRTIs.
Fig.2 Discovery strategies for HIV-1 NNRTIs. (Kang, et al., 2020)
Applications
- Drug development: Carbohydrate plays an important role in drug development, which improves the selectivity and efficacy of drugs through specific recognition and binding to target molecules. Glycoclick achieves the synthesis and modification of glycosyl drugs through controllable click reactions and chemical modifications, thereby improving the efficacy and bioavailability of drugs.
- Research on protein-glycosyl interactions: Specific interactions between sugar molecules and proteins are of great significance to cell signal transduction, immune response, and disease mechanisms. Click technology is used to synthesize functionalized glycosyl molecules, to study and analyze the interaction between glycosyl and protein, and to reveal the functions and mechanism of glycosyl in biological systems.
- Research on cell surface glycosyl modification: Glycosyl modification is one of the important features of the cell surface and affects biological processes such as cell adhesion, recognition, and communication. Click technology is used to customize compounds to study and explore glycosyl modification patterns on cell surfaces, providing new strategies and methods for disease research.
Advantages
- Professional scientific research team: CD BioGlyco has experienced and professional scientific research teams. they are proficient in using click technology to efficiently develop, optimize, and evaluate drugs.
- Efficiency: Glycoclick technology greatly improves the efficiency of drug development. Based on this technology, we carry out high-throughput screening, rapid drug synthesis, and optimization, thereby accelerating drug development.
- Diversity: Glycoclick technology is widely used in different drug development fields, including protein modification, antibodies, glycoproteins, etc. We apply click technology to provide various types of drug development services.
CD BioGlyco provides high-quality GlycoCLICK™-based drug development services. We respond quickly to market demand and flexibly adjust research directions and development strategies to meet clients' different drug development needs. Please feel free to contact us if you are interested in our development service.
References
- Jiang, X.; et al. Recent applications of click chemistry in drug discovery. Expert Opinion on Drug Discovery. 2019, 14(8): 779-789.
- Kang, D.; et al. In situ click chemistry-based rapid discovery of novel HIV-1 NNRTIs by exploiting the hydrophobic channel and tolerant regions of NNIBP. European Journal of Medicinal Chemistry. 2020, 193: 112237.
For research use only. Not intended for any clinical use.
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