GlycoCLICK™-based Enzymatic Lysis Service

GlycoCLICK™-based Enzymatic Lysis Service at CD BioGlyco

At CD BioGlyco, our GlycoCLICK™-based enzymatic lysis service focuses on the lysis and extraction of target molecules from cell or tissue samples. This allows rapid and efficient lysis of cells or tissues and extraction of target molecules for subsequent analysis and application. Our services apply to a wide range of your sample types, including cell cultures, animal tissues, and plant tissues. We help you perform high-throughput, high-efficiency, and high-quality cell or tissue lysis and target molecule extraction, providing vital support for your biological research and drug development. Here are the specifics of our service:

First, we prepare the cell or tissue samples to be enzymatically digested in advance, select the appropriate enzyme, and prepare the appropriate enzymatic buffer, according to the type of sample and the target molecule to be enzymatic lysis. The commonly used enzymes include lysozyme, lysostaphin, zymolase, cellulose, protease, or glycanase.
Next, we mix the sample with enzymatic buffer and perform the enzymatic reaction at the appropriate temperature and time. The enzyme will interact with specific components in the cell wall or cell membrane to rupture the cell wall or cell membrane and release the target molecules inside the cell. We then choose to stop the enzymatic reaction by changing the temperature or adding an inhibitor, depending on the circumstances. Subsequently, the target molecules after enzymatic lysis are separated and extracted using appropriate methods, such as centrifugation, filtration, or column chromatography.
Finally, the isolated target molecules are further analyzed for subsequent applications, including concentration and activity determination.

Fig.1 Flowchart of enzymatic lysis. (CD BioGlyco)

Publication Data

Technology: Cell lysis

Journal: Micromachines

IF: 3.523

Published: 2017

Results: This article presents a comprehensive overview of cell lysis methodologies. The article first introduces the importance and application areas of cell lysis, including molecular diagnostics, protein purification, cancer detection, and drug screening. Then, the article discusses two classifications of cell lysis: complete cell lysis and partial cell lysis. Complete cell lysis is employed for the examination of DNA, RNA, and subcellular constituents, whereas partial cell lysis is utilized for drug testing and the exploration of intracellular ionic currents. Next, the article describes macro-scale and micro-scale cell lysis methods. Macroscale methods include acoustic lysis and mechanical lysis, while microscale methods include enzymatic lysis and cell lysis using microfluidics. Enzymatic techniques are primarily utilized to solubilize lipids and proteins within cell membranes, facilitating the formation of pores and subsequent cell lysis. The application areas of enzymatic lysis in cell lysis include pathogen detection, nucleic acid analysis, and so on. Finally, the article provides a competitive analysis to aid in the selection of the appropriate cell lysis method for a given application.

Fig.2 Cell lysis using detergent to open the cell membrane and release the intracellular components. (Shehadul Islam, et al., 2017)

Applications

In molecular biology and genetics research, enzymatic lysis is used to extract DNA or RNA.
Enzymatic lysis is used to purify proteins by disrupting cell membranes and nuclei, releasing proteins, and helping to remove other cellular components.
In cell biology and immunology studies, enzymatic lysis is used to study the structure, function, and subcellular organization of cells. By disrupting cell membranes and cell walls, enzymes can make cells easier to observe and study.

Advantages

Enzymes enable hydrolysis by selectively targeting specific molecules or structures, thus allowing selective release of target molecules with greater specificity.
Enzymatic lysis is gentler and reduces thermal denaturation or damage to the target molecule, thereby better maintaining its integrity and activity.
Enzymatic lysis is suitable for a wide range of cell types and sample sources, including bacteria, plant cells, and animal cells.

For many years, CD BioGlyco has been offering simplified GlycoCLICK™-based Decoupling Service to our clients and it's been well received. We provide customized GlycoCLICK™-based enzymatic lysis through our extensive portfolio of services. Please feel free to contact us if you have any questions regarding our services.

Reference

Shehadul Islam, M.; et al. A review on macroscale and microscale cell lysis methods. Micromachines. 2017, 8(3): 83.

For research use only. Not intended for any clinical use.

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