Empower Your Research with GlycoCLICK™-based Cell Labeling

Cell labeling refers to the introduction of specific labels into a cell to identify, track, or detect the cell or specific molecules within it. By labeling cells or molecules within them, they can be localized and identified. By applying specific labels to the surface of a cell, it can be made to show different colors or fluorescent signals under a microscope, thus facilitating its observation and analysis by researchers. This technology is widely used in the biomedical field and also plays an essential role in basic science research, such as helping to reveal the mechanisms of cell development, differentiation, and function regulation. CD BioGlyco offers comprehensive GlycoCLICK™-based Labeling Service, enabling researchers to better understand the behavior and characteristics of these cells.

First, we culture the cells to be labeled in an appropriate medium to ensure cell health and growth. Then we introduce the labels into the cells using metabolic labeling agents such as N-azidoacetylmannosamine (Ac₄ManNAz). These labels usually bind to specific molecules on the cell surface, such as glycosylated glycoproteins. The copper catalyst is subsequently combined with an accelerator containing a reducing agent and introduced into the cells to facilitate the reaction at the optimal temperature. At the end of the reaction, we perform a thorough cell wash to eliminate any residual reactants and by-products. Subsequently, the cells are reintroduced into an appropriate culture medium to reinstate their growth and overall well-being. Following a designated period in culture, we evaluate the vitality and viability of the cells through established cell viability assays.

Fig.1 The process of cellular labeling. (CD BioGlyco)

Publication Data

Applications

• Labeling of specific molecules such as proteins, nucleic acids, etc. inside the cell can be achieved by introducing molecules containing click-responsive groups inside the cell to study their functions and mechanisms in terms of localization, translocation, and interactions inside the cell.
• It can be used to label specific molecules on the cell surface, such as glycosylated proteins, to study their function in cell signaling and cell-cell interactions.
• It is applied to track and localize cells by introducing visual markers, such as fluorescent dyes, into the cells.

Advantages

• It selectively labels molecules on the cell surface, such as glycosylated proteins, without affecting molecules inside the cells
• Cells can be monitored and precisely located by incorporating visual markers, such as fluorescent dyes, within the cellular structure.
• GlycoCLICK™-based cell labeling solutions enable fast reaction that allows for cell labeling in a short period and maintains cell viability.

At CD BioGlyco, we strive for continuous innovation by actively collaborating with academic institutions and industry partners worldwide. Furthermore, we provide tailored GlycoCLICK™-based cell labeling solutions that cater specifically to their unique requirements. Please contact us if you have any questions regarding our services.

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