GlycoCLICK™-based Labeling Service

GlycoCLICK™-based Labeling Service at CD BioGlyco

CD BioGlyco stands as a reputable entity in the field of glycochemistry, offering a plethora of technologies. With our team of proficient researchers, we aim to significantly expedite the research of our clients, ensuring a thorough and precise exploration in the realm of GlycoCLICK™-based Chemical Synthesis and labeling. By leveraging our extensive experience in GlycoCLICK™, CD BioGlyco is well-positioned to assist clients with GlycoCLICK™-based labeling services.

GlycoCLICK™-based Cell Surface Glycan Labeling Service

Cell surface glycan labeling is often used to study the role of glycan molecules on the cell surface in some important biological processes. We label the glycans on the cell surface by GlycoCLICK™ to help you with your research. Our GlycoCLICK™-based cell surface glycan labeling services are generally divided into two approaches, which are alkyny-based and azid-based cell surface glycan labeling.

GlycoCLICK™-based Antibody Labeling Service

CD BioGlyco offers advanced GlycoCLICK™-based antibody labeling services which alters the glycochain structure of antibodies, enabling more precise and effective labeling. We minimize the disturbance to the structure and function of your antibody samples through our highly selective labeling process. Our services are principally catered towards your research needs in fields like biomedicine and immunotherapy.

GlycoCLICK™-based Cell Labeling Service

Through our GlycoCLICK™ cell labeling service, we help researchers track the distribution and migration of cells throughout the body to better understand the progression of disease. Our GlycoCLICK™-based cell labeling service includes a wide range of cells such as mesenchymal stem cells (MSC), cancer cells, endothelial progenitor cells, and T cells.

Fig.1 The general process of GlycoCLICK™-based labeling services. (CD BioGlyco)

Publication Data

Technology: Fluorescence microscope, fluorescent labeling, and metabolic labeling

Journal: Frontiers in physiology

IF: 4.0

Published: 2014

Results: This article discusses specific and quantitative methods for biomolecular labeling using click chemistry. It is pointed out that the most commonly used quantitative bioanalysis method is the expression of foreign proteins using fused fluorescent tags. There are two challenges: the stoichiometry of precisely controlling chemical reactions in living cells and the volume of fluorescent labels limit the analysis of the inherent physical and biological properties of proteins. Click chemistry specifically and stoichiometrics label endogenous proteins or other biomolecules within cells. These chemical reactions take advantage of biological orthogonality, mild reaction conditions, high reaction ratio, small functional group size, stable covalent bonds, and simple metabolic labeling using various biomolecular analogs in living cells. These methods have been used in various studies in the field of quantitative biology, such as quantification of newly synthesized proteins and observation of post-translational modifications. Through click chemistry, quantitative fluorescence labeling of biomolecules can be realized for observing biological phenomena and studying systems biology.

Fig.2 Applications of click chemistry for biomolecular labeling. (Horisawa, 2014)

Applications

For protein imaging, GlycoCLICK™-based labeling enables direct observation and analysis of protein location, expression, interaction, and function by selecting and targeting fluorescent labels.
GlycoCLICK™-based labeling enables high-resolution imaging of cell membranes to observe and study their structure, dynamics, and interactions with other cellular structures and molecules.
GlycoCLICK™-based labeling technology can be used to label drug molecules to help researchers assess drug distribution, metabolism, and interactions, thereby facilitating drug discovery and efficacy evaluation.

Advantages

This technology is highly specific and enables precise attachment of fluorescent labels or other molecules to target molecules for highly specific labeling and imaging.
GlycoCLICK™-based labeling can be combined with a variety of biological and analytical methods, including flow cytometry, immunofluorescence staining, and mass spectrometry, to make it suitable to provide you with customized labeling services.
GlycoCLICK™ technology has a wide range of applications and it combined with different biomolecules, giving greater flexibility in your research.

At CD BioGlyco, our GlycoCLICK™-based labeling service is capable of handling a wide range of biological samples. Our optimized technology delivers high precision to help our clients move their projects forward more accurately and quickly. Please feel free to contact us to get the best in labeling services!

Reference

Horisawa, K. Specific and quantitative labeling of biomolecules using click chemistry. Frontiers in physiology. 2014, 5: 457.

For research use only. Not intended for any clinical use.

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