GlycoCLICK™-based Cell Surface Glycan Labeling Service

GlycoCLICK™-based Cell Surface Glycan Labeling Service at CD BioGlyco

Glycans on the cell surface are molecules with important biological functions, and they are involved in many biological processes, such as cell recognition, signaling, and disease occurrence. Cell surface glycan labeling is often used to study the role of glycan molecules on the cell surface in the process of cell recognition, signaling, and virus infection. Over the past years, CD BioGlyco has been working on research related to GlycoCLICK™ technology, including but not limited to GlycoCLICK™-based Chemical Synthesis, GlycoCLICK™-based Modification, and GlycoCLICK™-based Labeling. We provide you with professional GlycoCLICK™-based cell surface glycan labeling services based on two functional groups that are integral to the click reaction.

Azide-based Cell Surface Glycan Labeling Service

To better investigate the structure and function of cell surface glycans, we offer our clients azide-based cell surface glycan labeling service. By utilizing this technology, the azide group covalently binds with the cell surface glycan to form a stable label. This labeling is visualized, so it enables direct observation of the presence of glycans on the cell surface after labeling.

Alkynyl-based Cell Surface Glycan Labeling Services

We provide professional alkyne-based cell surface glycan labeling services. We introduce glycan-containing alkyne groups to the cell surface. Then the probes containing fluorescence or biotin are attached to the glycan on the cell surface by click chemistry to achieve the labeling purpose. Our method enables precise labeling of cell surface glycan molecules while avoiding damage to your cell samples.

Fig.1 GlycoCLICK™-based cell surface glycan labeling. (CD BioGlyco)

Publication Data

Technology: Metabolic oligosaccharide engineering (MOE)

Journal: Angewandte Chemie

IF: 15.336

Published: 2013

Results: This article mainly discusses a combination of two chemical methods for the labeling of glycans in living cells. The researchers used the Diels-Alder reaction and click chemistry (SPAAC) to simultaneously detect two different metabolic sugars. By adding Ac₄ManNPtl, which contains an olefin label, and N-acetyl compounds, which contain an N-azidoacetyl label, to HeLa S3 cells, they successfully labeled two different sugars on the cell surface. The researchers also found that the olefin label had less background staining in the cells and that the non-specific staining could be due to non-specific binding or the addition reaction of the SH group to the triple bond. In addition, the researchers found that this method can be used simultaneously with sugars containing alkyne labels, enabling simultaneous detection of two different sugars. This method is important for advanced multicarbohydrate analysis. In addition, because olefin labeling can also be easily accepted by other biomolecules (such as proteins), this labeling method is not only suitable for metabolic glycan engineering but also can be widely used in other fields.

Fig.2 Dual-labeling strategy for MOE employing alkene- and azide-labeled monosaccharides. (Niederwieser, et al., 2013)

Applications

GlycoCLICK™-based cell surface glycan labeling can be used to label and visualize glycans on cell surfaces for the development of pathological diagnosis methods.
GlycoCLICK™-based cell surface glycan labeling is used in biomedical research, e.g. to analyze the interactions between cell surface glycans and other biomolecules.
It is used to specifically label a certain glycan on the surface of a cell and to study its role in the development of a disease, leading to new drug therapeutic options.

Advantages

Modified glycan precursors are linked by click chemistry to fluorescent molecules or biotin with high sensitivity and efficiency.
Our technology applies to a wide range of cell types and biological samples, including primary cells, cell lines, and tissue sections.
GlycoCLICK™-based cell surface glycan labeling is highly specific and can be targeted to study cell surface glycans.

CD BioGlyco possesses advanced technologies and systematic solutions to provide comprehensive GlycoCLICK™-based cell surface glycan labeling service. We put our utmost efforts into assisting our clients with cell surface glycan labeling, and ensuring that we deliver top-notch service to our clients. Please feel free to contact us if you have any questions regarding our services.

Reference

Niederwieser, A.; et al. Two-color glycan labeling of live cells by a combination of Diels-Alder and click chemistry. Angewandte Chemie. 2013, 52(15): 4265–4268.

For research use only. Not intended for any clinical use.

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