Trans-cyclooctene (TCO)/Tetrazine (Tz) Ring-based Click Imaging Service
Unlocking Molecular Interactions with Trans-cyclooctene (TCO)/Tetrazine (Tz) Click Imaging
TCO/Tz ring-based click imaging employs TCO and Tz compounds possessing specific structures for click reactions, resulting in the formation of stable conjugated products suitable for imaging. Through the synthesis of appropriate TCO and Tz markers and their application to biological samples, highly sensitive imaging of targeted molecules, cells, or tissues can be achieved. The utilization of TCO/Tz ring-based click imaging has gained significant recognition in various biomedical applications encompassing cellular imaging, tissue imaging, as well as in vivo imaging. CD BioGlyco has been providing clients with specialized GlycoCLICK™-based Imaging Service for many years, including Azide (Az)/Difluorocyclooctene (DIFO)-based Click Imaging Service, Az/Dibenzylcyclooctyne (DBCO)-based Click Imaging Service and TCO/Tz ring-based click imaging service.
- We help accelerate your research in areas such as drug discovery and development by utilizing TCO/Tz click reactions to label biomolecules or drugs, which can then be viewed in real-time and quantified by in vivo imaging techniques such as positron emission tomography (PET) or single photon emission computed tomography (SPECT).
- Our experts apply the TCO/Tz click reaction at the cellular level, helping you to investigate intracellular molecular interactions, signaling pathways, etc., and to observe dynamic processes in the cell with the help of fluorescence microscopy and other techniques.
- We use the TCO/Tz click reaction in tissue samples to enable imaging of tissue structure and protein distribution. By using specific tagging agents, we visualize different types of proteins in tissue samples and learn more about their distribution at the cellular and tissue levels.
- TCO/Tz click reaction is employed to label drugs or nanocarriers, utilize imaging techniques to monitor drug release and distribution in vivo and evaluate the efficacy and targeting of drug delivery systems.
- By employing click reaction marker molecules, we facilitate the visualization of molecular interactions, complex formation, and other processes. This approach enables a comprehensive understanding of biological mechanisms at the molecular level.
Fig.1 TCO/Tz ring-based click imaging service. (CD BioGlyco)
Publication Data
Technology: PET and SPECT-computed tomography (CT) imaging
Journal: Bioconjugate Chemistry
IF: 4.7
Published: 2019
Results: This article describes an antibody pre-labeling method based on bioorthogonal click chemistry for cancer imaging and targeted radiotherapy. Studies of antibody pre-labeling using the inverse-electron-demand Diels-Alder cycloaddition (IEDDA) TCO/Tz cycloaddition reaction are mentioned, and some relevant experiments in mouse models are cited. By optimizing the two components of the click reaction, immobilization on the tumor is maximized. In vivo studies using TCO/Tz IEDDA pre-targeting mainly report two Tz primers, bis(pyridyl)-1,2,4,5-tetrazole and benzyl-1,2,4,5-tetrazole. These two Tz primers rapidly accumulate in tumors within 24 hours to several days (120 hours) after injection of TCO-mAbs and are cleared rapidly, allowing for good signal/background noise during imaging and low toxicity during radionuclide therapy. The article also mentions potential application areas for the method, including modification of nanoparticles, biosynthesis of antifungal drugs, and labeling of HIV-1 envelope glycoproteins. However, despite some encouraging results in recent years, the method has not yet reached the clinical trial stage and there may be some potential obstacles to overcome.
Fig.2 Depiction showing the release of a drug bound to the antibody-drug conjugate. (Rondon & Degoul, 2019)
Applications
- TCO/Tz ring-based click imaging can be used to study biological processes such as protein interactions, cell signaling pathways, and protein function. Labeling molecules by this click chemistry method enables the study of localization and dynamic changes of biomolecules in living cells or living models.
- TCO/Tz ring-based click imaging can be used to study the distribution, metabolic pathways, and targeting effects of drug delivery systems, and nanomedicine carriers in vivo.
- In cell tracking and immunology studies, TCO/Tz ring-based click imaging can be used to label and track specific cell types, migration, and aggregation of immune cells.
Advantages
- We integrate pre-targeting strategies with cutting-edge advancements in molecular fluorescent dye probes to offer enhanced contrast imaging.
- We not only provide imaging technology, but also offer services such as experimental program design, data analysis, and other tailored solutions to help clients fully leverage the benefits of this technology.
- The TCO/Tz ring click imaging is not only applicable to antibodies but also to other systems such as organic molecules, peptides, antibody fragments, nanoparticles, or polymer micelles.
At CD BioGlyco, our state-of-the-art TCO/Tz ring-based click imaging service distinguishes us from conventional methods by providing a more efficient and streamlined approach to drug discovery and development. If you would like more detailed information, please feel free to contact us directly.
Reference
- Rondon, A.; Degoul, F. Antibody pretargeting based on bioorthogonal click chemistry for cancer imaging and targeted radionuclide therapy. Bioconjugate Chemistry. 2019, 31(2): 159-173.
For research use only. Not intended for any clinical use.
Related Services
