Revolutionize Your Research with Innovative Carbohydrates Click Chemistry

Lectins are carbohydrate-specific proteins that act as recognition molecules in various biological systems. These proteins bind reversibly to carbohydrates and are highly specific for monosaccharides/oligosaccharides and are also known as glycan-binding proteins. CD BioGlyco utilizes Cu(I)-assisted 1,3-dipolar azide-alkyne cycloaddition (CuAAC) reactions to provide development services for different reagents through different strategies.

Here, we utilize click chemistry methodology to provide a trusted carbohydrate click-based lectin ligand development service.

• We use aromatic compounds and aldehydes as the main sources for the two-step preparation of stereoselective aromatic compounds. We characterize them using nuclear magnetic resonance techniques and infrared spectroscopy.
• Next, we couple aryl hydroxylated glycosides to azide-functionalized glycosides via Cu(I)-assisted 1,3-dipolar azide-alkyne cycloaddition (CuAAC) to obtain a high yield of aryl hydroxylated glycoclusters, which are used as ligands for subsequent binding to the lectin receptor. In this process, the CuAAC reaction is characterized by high efficiency and yield and avoids incomplete reaction of alkyne groups.
• Then, we bind the prepared glycoclusters to the carbohydrate epitopes of lectins to research the binding effect of glycoclusters on lectins. Among them, we provide hemagglutination inhibition assay (HIA), enzyme-linked lectin assay (ELLA), and surface plasmon resonance technique (SPR) to research the binding affinity of glycocluster to lectin.

• HIA: We separately co-incubated different amounts of lectin solution with diluted glycoclusters. After some time, erythrocyte solution is added to it and the incubation is continued. Finally, the lowest inhibitory concentration of each glycocluster molecule is determined.
• ELLA: We use carbonate buffer solution to dissolve the glycocluster ligand and add biotinylated lectin solution to it for co-incubation. After some time, the peroxidase coupling material is added and the absorbance is measured to determine the semi-inhibitory concentration of the glycocluster ligand.
• SPR: A biotinylated glycan solution is applied to the surface of the lectin by streptavidin-biotin interaction, followed by inhibition experiments.

• Furthermore, we provide molecular modeling to research the binding sites of glycocluster and lectin. Combined with the use of fluorescent labeling, the exact location of the glycocluster as it binds to the surface of the lectin is determined in a model of the lectin's steric structure.

Fig.1 Process of developing lectin ligands based on carbohydrate clicks. (CD BioGlyco)

Publication Data

Applications

• Carbohydrate click-based lectin ligand development can be used to probe in depth the specific binding properties of proteins and glycans.
• Carbohydrate click-based lectin ligand development can be used to assist in the development of lectin inhibitors.
• Carbohydrate click-based lectin ligand development can be used in the development of lectin-based drug substrates.

Advantages

• We have a first-class lectin ligand R&D technology and R&D team, and our scientists provide custom lectin ligand R&D solutions for each client.
• We combine click chemistry with lectin ligand R&D to provide a more efficient R&D route to produce high-quality and stable lectin ligand products.
• We have comprehensive pre-sales and after-sales services to provide timely project progress to our clients and ensure their tracking and participation in the project.

With first-class click chemistry technology, CD BioGlyco provides high-quality GlycoCLICK™ services to our clients. Meanwhile, we combine click chemistry with drug development to provide more efficient and stable Drug Development Services. If you are interested in our services, please feel free to contact us.

• GlycoCLICK™-based Enzyme Inhibitor Development Service