GlycoCLICK™-based Triazole Skeleton Molecule Imaging Service
GlycoCLICK™-based Triazole Skeleton Molecule Imaging Service at CD BioGlyco
At CD BioGlyco, our team leaders with years of experience in the GlycoCLICK™-based Imaging Service oversee all projects, and our operational support and specialists are on hand at every stage of the project to drive success and minimize problems. We offer a one-stop solution for your triazole skeleton molecule imaging project, helping to make it a success in a variety of ways. We fully understand that every GlycoCLICK™-based triazole skeleton molecule imaging project we offer needs to be different, and therefore we provide tailored services to meet the specific needs of our clients.
Covalently labeled radionuclide imaging service
First, we design and synthesize the appropriate labeling molecule for your needs and ensure that it has sufficient specificity and affinity to bind to the biomolecule or tissue to be imaged. The metal radionuclide is then efficiently labeled on the nanoparticles, which are then covalently labeled with 125I by chemical substitution of hydrogen atoms in the benzene ring. Subsequently, the radiotracer is validated and quality-controlled by checking parameters such as purity, activity, stability, and toxicity. In the final stage, a series of pertinent imaging experiments are conducted to capture the required data. Following this, the obtained imaging data is carefully examined, analyzed, and interpreted using advanced techniques. The comprehensive findings and observations are then consolidated into a detailed imaging report.
Metal chelates-based radioactive tracers imaging service
First, we prepare an organic molecule capable of forming a stable complex with a metal ion, often containing multiple electron-donating atoms such as nitrogen or oxygen. Secondly, we convert the chosen metal element into a radioisotope and an ionic form. Thirdly, we react to the radioactive metal ions with precursors to form radioactive chelates and test their stability and affinity to target molecules in organisms. Later, we injected the chelates into animal model pinches to distribute them in vivo, especially to accumulate in the target tissues or organs that need to be observed. Techniques such as positron emission tomography (PET) and single photon emission computed tomography (SECT) are used to detect and image the distribution and movement of the radioactive metal chelates in vivo. Finally, the imaging results are analyzed and a report is generated.
Fig.1 Applications of radionuclide labeled nanomaterials. (CD BioGlyco)
Publication Data
Technology: PET and SECT
Journal: Biomaterials Science
IF: 6.6
Published: 2021
Results: This article describes a multi-radioisotope labeled metal-organic nanoframework (MOF) for tumor imaging and radioisotope therapy. The researchers achieved multimodal imaging of tumors, including PET and single photon emission computed tomography (SPECT), by labeling different radioisotopes onto the MOF. In the study the authors synthesized PCN-PEG nanoparticles by modifying zirconium-based nMOF nanoparticles (PCN-224) with polyethylene glycol (PEG) and modifying carboxylic acid groups on the surface of PCN-PEG. These carboxylic acid groups were then used to covalently attach to different radionuclides, including 177Lu, 99mTc, and 125I, to achieve radiolabelling by a simple and efficient reaction. In cellular experiments, 177Lu-PCN-PEG nanoparticles were efficiently taken up by cells and caused apoptosis. In mouse models, 177Lu-PCN-PEG nanoparticles were able to achieve high accumulation in tumors, significantly inhibit tumor growth, and prolong the survival time of mice. Therefore, PCN-PEG nanoparticles have potential applications in tumor diagnosis and therapy.
Fig.2 Schematic illustration of versatile labeling of multiple radionuclides onto a nanoscale metal-organic framework (PCN-PEG) for tumor imaging and radioisotope therapy. (Tao, et al., 2021)
Applications
- By covalently labeling radioactive tracers onto specific molecules, it is possible to achieve highly selective imaging against relevant receptors or antigens on the surface of tumor cells. This helps to detect the presence, location, and size of tumors.
- Covalently labeled radionuclide imaging can be used to observe the distribution and activity of neurotransmitters in real-time when studying the structure and function of the nervous system.
- Covalently labeled radionuclide imaging can be used to track the metabolism, distribution, and excretion of drugs in the body.
Advantages
- Metal chelates can be used not only as radiotracers but also in combination with non-radioactive markers such as fluorescent dyes for multimodal imaging, providing more comprehensive information for your research.
- The structure of metal chelates can be modulated by chemical synthesis, and metal chelates with different affinity and biodistribution properties can be designed to meet different imaging needs.
- Covalently labeled radiotracers can be monitored and tracked in real-time by imaging equipment, providing more detailed information and data.
CD BioGlyco has advanced nanoparticle production technologies and flexible solutions. Our experts develop customized GlycoCLICK™-based triazole skeleton molecule imaging protocols to perform, maintain, and deliver your project with a high degree of flexibility and transparency. Please feel free to contact us if you have any questions regarding our services.
References
- Tao, Y.; et al. Versatile labeling of multiple radionuclides onto a nanoscale metal-organic framework for tumor imaging and radioisotope therapy. Biomaterials Science. 2021, 9(8): 2947-2954.
- Boros, E.; Holland, J.P. Chemical aspects of metal ion chelation in the synthesis and application antibody-based radiotracers. Journal of Labelled Compounds and Radiopharmaceuticals. 2018, 61(9): 652-671.
For research use only. Not intended for any clinical use.
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