Empower Your Immunology Research with Advanced T Cell Labeling
Metabolic labeling is a technique employed in Cell Surface Labeling by utilizing biomolecules derived from the metabolic processes of the cell. Click chemistry, on the other hand, refers to a specific chemical reaction that can occur within an organism or on the cell surface and is utilized for covalently binding two molecules together. CD BioGlyco integrates metabolic labeling with click chemistry to offer clients a comprehensive T cell labeling service.
- First, we metabolically label T cells using N-azidoacetylmannosamine-tetraacetylated (Ac4ManN3). We introduce the azide moiety onto the cell surface by supplementing the cell culture medium with azide-functionalized sugars, which are internalized by the cells and subsequently react with molecules residing on their surface.
- After culture and successful metabolic labeling of T cells, we use sulfo-6-methyl-tetrazine-dibenzyl cyclooctyne (TzDBCO) to react with metabolically labeled T cells via the strain-promoted alkyne-azide cycloaddition (SPAAC) reaction. The SPAAC reaction is a highly efficient biological orthogonal click reaction that occurs through the reaction between azide and cyclooctyne to form a stable triazole bond.
- Finally, the trans-cyclooctene (TCO) functionalized nanoparticles are utilized for the reaction with SPAAC-reacted T cells to form complexes that are covalently bonded.
Fig.1 Process of T cell labeling. (CD BioGlyco)
Publication Data
Technology: Metabolic labeling
Journal: Angewandte Chemie International Edition
IF: 16.823
Published: 2021
Results: This paper explores an approach that utilizes a combination of metabolic labeling and click chemistry to covalently bind nanoparticles to the cell surface to enable targeted drug delivery for specific cell types. The researchers used a combination of metabolic labels and click chemistry to achieve this. They first introduced azide sugar to the cell surface via metabolic labeling and then used the SPAAC to covalently bind metabolically labeled T cells to fluorescent dyes containing DBCO functions. This covalent binding allows the nanoparticles to bind firmly to the T cell surface, enabling targeted drug delivery for specific cell types. This paper also discusses the problems of non-covalent and covalent methods of binding nanoparticles on the cell surface and introduces the experimental results of binding lipid nanoparticles containing polycreatine polynucleotides to the cell surface by this method.
Fig.2 Approaches of cell surface bioorthogonal conjugation in this study. (Lamoot, et al., 2021)
Applications
- T cell labeling technology can be used in the investigation of T cell functionality and biological mechanisms.
- Labeling T cells with fluorescent dyes or radioisotopes, for example, can be used to track their localization and migration within the body and to study their interactions with other immune cells and their immune response to pathogens.
- Researchers employ T cell labeling techniques to monitor and assess their in vivo functionalities, encompassing their capacity to identify and combat malignant cells.
Advantages
- It enables highly specific cell surface binding and reduces non-specific background binding.
- This technology allows for visual visualization, by labeling T cells with specific fluorescent dyes or radioisotopes and tracking their movement and activity in the body.
- By labeling T cells, it is feasible to distinguish them from other cells or markers, thus improving the accuracy and reproducibility of experiments.
CD BioGlyco provides a comprehensive T cell labeling solution, catering to the diverse needs of your research project and ensuring its triumphant accomplishment. Please feel free to contact us for more details of our service, we are always available to answer any questions you may have.
Reference
- Lamoot, A.; et al. Covalent cell surface conjugation of nanoparticles by a combination of metabolic labeling and click chemistry. Angewandte Chemie International Edition. 2021, 60(12): 6320-6325.
For research use only. Not intended for any clinical use.
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