Azide-based Ribose Modification Service
Precision and Quality Assured Azide-based Ribose Modification at Your Fingertips
In recent decades, CD BioGlyco has expanded our capabilities on a global scale, offering researchers comprehensive GlycoCLICK™-based Modification Service. In particular, our azide-based ribose modification service has received favorable reviews. Azide-based ribose modification is usually a cyclization reaction of an azide group with an alkyne group on a ribose to form a stable ring compound. The reaction allows selective modification in the RNA molecule.
Typically, since free ribose is usually present at the 3'-terminus of RNA, it is easy to react with modification reagents. Therefore, our azide-based ribose modification service focuses on modification at the 3'-end of RNA molecules. The specific reaction site selection and modification efficiency are affected by a variety of factors, including the structure and concentration of the modification reagent, the optimization of reaction conditions, and the structure and sequence of the RNA molecule. Therefore, we carefully consider these factors when designing and conducting azide-based ribose modification experiments, and optimize and adjust accordingly for your specific experimental purposes.
To begin with, we synthesize nucleotide precursors containing azide groups. Following that, based on the experimental design and requirements, we prepare the desired target molecules through chemical synthesis, transcription, or cell extraction methods. Next, we combine the synthesized azide-modified nucleoside with the target ribose molecule, subjecting them to suitable conditions for a reaction. Lastly, we purify the resulting modified products and confirm their structure and purity through techniques such as mass spectrometry analysis and nuclear magnetic resonance (NMR).
Publication Data
Technology: Chemical modification
Journal: Molecules
IF: 4.927
Published: 2021
Results: This article focuses on the synthesis of improved nucleotides, nucleosides, and nucleic acids via click-based azide-alkyne cycloaddition reactions and explores their potential applications in pharmaceutical applications. The article also describes the principles and advantages of the click reaction and describes in detail the specific steps involved in the synthesis of improved nucleotides and nucleic acids. The synthesis of modified nucleotides and nucleic acids primarily involves the utilization of azide-alkyne cycloaddition reactions. Under commonly used reaction conditions, such as (1:1:1.5) H2O/t-BuOH/THF (v/v) system, alkyne nucleotides or nucleoside derivatives were subjected to click reaction with azide reactive groups in the presence of CuSO4 catalyst and sodium ascorbate at room temperature. The reaction time was 18 hours. Finally, different modified nucleotides and nucleosides were synthesized by synthesizing different combinations of alkynyl nucleotides or nucleoside-like derivatives and azide reactive groups as required.
Fig.1 Molecular structure alkyne- or azide functionalized nucleosides. (a) commercially compounds; (b) reported compounds. (Perrone, et al., 2021)
Applications
- By introducing ribose analogs containing azide groups, selective labeling is introduced into RNA at specific locations, which is applied to photocontrol or fluorescent labeling of RNA to facilitate the study of its dynamic behavior and interactions.
- Azide-based ribose modifications are used in the research and regulation of protein synthesis by introducing specific functional groups or fluorescent tags into the protein being synthesized.
- Azide-based ribose modifications can be used in the design and development of drug molecules. By introducing ribose analogs containing azide groups, click reactions with specific drug molecules can be carried out to achieve targeted delivery, release, or activation of drug molecules.
Advantages
- Our azide-based ribose modification technology usually features high modification efficiency and reaction speed and can achieve ribose modification in a relatively short time.
- Azide-based ribose modification is compatible with a wide range of reagents and reaction conditions, which extends its application in many areas.
- Azide-based ribose modification can be used not only for research on RNA structure, function, and interaction but also for the construction of biomaterials with specific functions, as well as the design and development of drug molecules and other fields.
CD BioGlyco provides outstanding azide-based ribose modification services to meet your needs. Our team of experienced professionals is readily available to assist you with any inquiries or concerns you may have. We are dedicated to serving you and supporting the advancement of your research. If you need further details of our service, please don't hesitate to contact us.
Reference
- Perrone, D.; et al. Modified nucleosides, nucleotides and nucleic acids via click azide-alkyne cycloaddition for pharmacological applications. Molecules. 2021, 26(11): 3100.
For research use only. Not intended for any clinical use.
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