GlycoCLICK™-based Oligosaccharide Synthesis Service

GlycoCLICK™-based Oligosaccharide Synthesis Service at CD BioGlyco

CD BioGlyco provides an efficient click chemistry-based oligosaccharide synthesis service. We use various types of click chemical reaction strategies to synthesize diverse oligosaccharides, such as C-disaccharide, S-disaccharide, and O-disaccharide. Click reactions are highly specific and efficient, and have broad application prospects in glycochemistry research and drug discovery. Our service includes but is not limited to the following.

Oligosaccharide sequence design service

We design the oligosaccharide sequence to be synthesized according to client needs and goals, including selecting the required monosaccharide units, quantity, connection sequence, and selection of protecting groups.

Oligosaccharide synthesis service

We use click chemistry to synthesize oligosaccharides with different sequences and structures. This method has the advantages of high efficiency, controllability, and flexibility, which is an important application tool in the field of glycochemistry.

Preparation of vector	We select appropriate vectors for preparation, such as alkynyl-modified molecules or solid-phase vectors. The alkynyl group on the vector undergoes a click reaction with the azide group.
Modification and synthesis of sugar monomers	We use organic chemistry methods to synthesize the desired sugar monomers that contain functional groups, which react with click reaction sites, such as sugar monomers containing azide group.
Click reaction	We perform a click reaction of an alkynyl-modified vector with an azide donor of the first monosaccharide unit. The reaction is carried out with an appropriate copper catalyst so that the first monosaccharide unit is attached to the vector.
Repeat click reaction	We repeat the steps of the click reaction, stepwise adding the required number of sugar monomers, to build the oligosaccharide of the desired length. In each click reaction, a new monosaccharide unit of the azide donor is used to click with the oligosaccharide chain already attached to the vector. Thus, the target oligosaccharide sequence is gradually constructed.
Deprotection	After the click reaction is completed, we select the appropriate deprotection method and perform the deprotection step under appropriate conditions.

Efficient purification service

We provide efficient purification for synthesized products to obtain high-purity oligosaccharide samples. Purification methods include column chromatography, solvent crystallization, and other techniques to remove impurities and isolate the target product.

Structural characterization service

We use a variety of analytical techniques (such as mass spectrometry, nuclear magnetic resonance, and high-performance liquid chromatography) to perform structural characterization and confirmation for synthesized oligosaccharides, which helps to verify the consistency of the synthesized oligosaccharides with the target sequence.

Quantitative analysis service

We measure the content of oligosaccharide samples through quantitative analysis techniques such as colorimetry and spectroscopy to ensure accurate oligosaccharide concentration information.

Fig.1 GlycoCLICK™-based oligosaccharide synthesis service. (CD BioGlyco)

Publication

Technology: A thio-click approach

Journal: Carbohydrate Research

IF: 2.975

Published: 2018

Results: The authors synthesized a series of 4-thiomalto-oligosaccharide compounds up to the pentasaccharide level through sequential light-initiated thiol-ene coupling reactions, in which each α-S-glycosidic bond was constructed through a thiol-ene coupling reaction, and has unique selectivity. The thio click reaction avoided the use of complex protecting group strategies during synthesis and had become a valuable tool for the synthesis of carbohydrate derivatives.

Fig.2 Synthesis of the α-S-linked pentasaccharide. (Lázár, et al., 2018)

Applications

Oligosaccharide drug development: Oligosaccharides have a wide range of biological activities and pharmaceutical potential. Through click chemistry, oligosaccharides with specific structures are custom synthesized and used in the field of drug discovery and development.
Glycoprotein research: Glycoproteins are complexes of sugar molecules attached to proteins that are critical to many biological processes. Synthesis of oligosaccharide chains with specific glycosyl modifications facilitates the study of glycoprotein function and interactions.
Glycobiology research: Carbohydrates play an important role in cell recognition, signaling, and immune response. By custom-synthesizing oligosaccharides with specific glycosyl structures, researchers explore sugar-related biological processes.

Advantages

CD BioGlyco has technical experts in the fields of glycochemistry, biosynthesis, and click chemistry, and conducts custom synthesis of oligosaccharides according to client needs.
We employ strict quality control procedures and use advanced analytical techniques to verify the accuracy and purity of our synthetic products. Our synthesized oligosaccharides are of high purity and good quality.
We have a variety of glycosyl modification methods to synthesize different types and diversity of sugar structures to support research needs in various fields.

CD BioGlyco provides clients with high-quality custom GlycoCLICK™-based oligosaccharide synthesis service. We develop and apply new synthesis methods and strategies to complete the synthesis of oligosaccharides within a reasonable time and deliver them to clients on time. Please feel free to contact us if you would like to obtain high-quality oligosaccharides quickly.

Reference

Lázár, L.; et al. Synthesis of thiomaltooligosaccharides by a thio-click approach. Carbohydrate Research. 2018, 470: 8-12.

For research use only. Not intended for any clinical use.

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