GlycoCLICK™-based Modification Service
GlycoCLICK™-based Modification Service at CD BioGlyco
CD BioGlyco has extensive experience in providing customized GlycoCLICK™-based Chemical Synthesis and modification services. Our professional research team and optimized experimental process provide clients with high-quality GlycoCLICK™-based modification services, which include surface modification, protein modification, and ribose modification.
Our GlycoCLICK™-based ribose modification service includes azide-based ribose modification service and alkynyl-based ribose modification services.
For surface modification, we usually perform click reactions directly on the surface, such as introducing azide or alkyne groups on the silicon surface through silane coupling reaction, and then modify them through click reaction. For protein modification, we modify fluorescent probes, biotin, or other functional groups onto proteins via click reactions. This involves introducing an alkyne group into an amino acid molecule, covalently bonding with the protein, and then grafting a functional group at that position via click reaction. As for ribose modification, we mainly use the azide-alkyne Huisgen 1,3-bicycloaddition reaction to specifically link ribose (or ribose derivatives) containing the azide moiety to labels (e.g. fluorophores, biotin, etc.) containing the alkyne moiety. This method is an important tool for modifying single or multiple nucleic acid molecules (e.g., RNA) for applications such as investigating their structure and function, or developing novel diagnostic reagents and drugs, because of its excellent chemoselectivity and ideal reaction conditions. We choose the technology and develop the solution for you according to your specific needs and research objectives.
Fig.1 Flow chart of GlycoCLICK™-based surface modification service. (CD BioGlyco)
Publication Data
Technology: Metabolic labeling, DNA assembly technologies, and click fixation techniques
Journal: Chemical Reviews
IF: 72.087
Published: 2021
Results: This article focuses on the application of nucleic acid click chemistry and related guidelines, including copper-catalyzed azide-alkyne cycloaddition (CuAAC), ruthenium-catalyzed click reactions (RuAAC), and metal-independent click reactions (SPAAC) techniques. Among them, CuAAC technology is widely used in nucleic acid modification and synthesis, such as the synthesis of large double-stranded DNA rings, triple-stranded and quadruple-stranded DNA structures by CuAAC. The article mentions that DNA double strands synthesized by click chemistry can be slightly unstable in some cases, but the stability of DNA double strands can be increased by introducing triazole groups. The article also discusses the effect of the combination of triazole linkage and mismatch sites on the stability and base stacking of DNA double strands. In addition, the article describes the use of click chemistry in antisense oligonucleotides (ASOs) and the enhancement of base-pairing stability through the introduction of G-clamp or sugar moiety modifications. The article also mentions the use of RuAAC and SPAAC for the synthesis of triazole-modified nucleic acids. In addition, some new findings and innovations are presented in this article, such as cGAMP derivatives synthesized via clicks have a more open conformation, which may be more favorable for interaction with cyclase cGAS. Another new finding is that DNA nanostructures synthesized by click synthesis can be stably prepared and purified under denaturing conditions.
Fig.2 Alternative strategy to Sonogashira coupling for the modification of nucleosides with alkyne functionalities. (Fantoni, et al., 2021)
Applications
- GlycoCLICK™ technology labels glycan structures on cell surfaces or proteins, helping researchers understand the role of glycosylation in cell signaling, disease development, and other processes.
- GlycoCLICK™ technology is used in the research of protein modifications such as glycosylation, phosphorylation, methylation, and other modifications, helping to reveal the effects of these modifications on protein function and interactions.
- GlycoCLICK™ is used to develop new biomolecular probes for the detection, screening, and visualization of specific glycosylated protein or nucleic acid sequences, enabling the study and monitoring of specific biological processes.
Advantages
- GlycoCLICK™ technology introduces specific glycan groups or other modifying groups to the surface of the target molecule through a suitable chemical reaction, which is highly selective.
- The technology applies to multiple fields of research, providing customized solutions for clients' research.
- Our experienced and professional technical team is familiar with the GlycoCLICK™ technology and provides professional consultation and technology to our clients.
CD BioGlyco has established a comprehensive GlycoCLICK™-based modification procedure to assist our clients in investigating the intimate correlation between click modification and precision medicine. We offer our clients premium quality, economically efficient, and hassle-free services. Please feel free to contact us for further detailed information if you are interested in our services.
Reference
- Fantoni, N.Z.; et al. A hitchhiker's guide to click-chemistry with nucleic acids. Chemical Reviews. 2021, 121(12): 7122-7154.
For research use only. Not intended for any clinical use.
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