Truncated Glycan Tn Antigen Vaccine Development Service
Complete Strategies for Truncated Glycan Tn Antigen Vaccine Development
CD BioGlyco has developed an advanced GlycoCLICK™-based Vaccine Development platform, providing high-quality Triazole Skeleton-based Vaccine Development, Carbohydrate Click-based Sugar-conjugated Vaccine Development, and Multivalent Glycopeptide-lipopeptide Vaccine Development services. Mucin type O-glycome expresses the truncated glycans Tn and STn in cancer cells. Truncated Tn antigen is a key target for anti-cancer vaccine development. It is an N-acetylgalactosamine (GalNAc) residue linked to a serine or threonine residue of mucin (GalNAcα1-Ser/Thr). At CD BioGlyco, we provide truncated glycan Tn antigen vaccine development services including design and synthesis, Tn antigen modification, animal model construction, immunization schedule, and immune assessment.
- Design and Synthesis Service
We provide fully synthetic vaccine development service with high truncated glycan Tn-antigen valency. Our researchers achieve modular polymeric assembly by copper(I)-catalyzed alkyne-azide cycloaddition (CuAAC) click chemistry. The building blocks we provide show protected Tn-Ser residues and some free amino groups, and the intermediates are obtained by coupling the building blocks to the N-hydroxysuccinimide (NHS) ester of Boc-(S-(3-nitro-2-pyridinesulfonyl)]-cysteine (Boc-Cys(NPys)-NHS) in N, N-dimethylformamide (DMF). In addition, we design different alternative strategies to optimize the vaccine structure. We use 1,4-triazole instead of internal linkage to optimize the structure. We also provide purification services of the syntheses by high-performance liquid chromatography (HPLC).
- Tn Antigen Modification Service
Modification of glycan antigen structure to make them more unfamiliar to the host improves the immunogenicity of carbohydrate vaccines. Therefore, we offer Tn antigen modification services such as N-acyl modification and fluorinated modification. For the modified vaccine, we further provide activity assessment service.
- Biological Activity Test Service
For synthetic vaccines, we offer services to evaluate their ability to respond due to their immune response. Our professional staff helps clients plan immunization cycles and construct animal models. In addition, we offer serological analysis,enzyme-linked immunosorbent spot (ELISPOT) assay, enzyme-linked immunosorbent assay (ELISA) analysis, and flow cytometry analysis.
Publication
Technology: Chemoselective click-type chemistry, Flow cytometry, Indirect immunofluorescence
Journal: Chemical science
Published: 2020
IF: 8.4
Results: In this work, we successfully synthesized a hexadecavalent Tn vaccine candidate. We used triazole groups to replace four internal oxime linkers to synthesize alternative Tn-Ser structures using CuAAC click chemical polymerization assembly modules. These Tn-based structures involve an effective solution to the problem of promoting B-cell activation. In addition, the new vaccine effectively induced Tn-specific humoral and CD4+/CD8+ cellular responses. The efficient immune properties of this fully synthetic vaccine make it a strong candidate for anti-cancer vaccines.
Fig.1 Cell surface reactivity of mouse antisera against native Tn antigen-expressed on the surface of human breast cancer cell line, MCF7. (Pifferi, et al., 2020)
Applications
- Vaccines developed based on truncated glycan Tn antigen are effective targets for cancer therapy study such as colon, bladder, cervical, ovarian, gastric, and prostate cancers.
- Truncated glycan Tn antigen plays an important role in immunological therapeutic applications. It is a very ideal drug candidate.
- Vaccines consisting of a variety of clustered Tn antigens and Th-cell peptide epitopes induce strong and durable Tn-specific responses with IgM/IgG antibodies and produce significant antibody-dependent tumor regression.
Advantages of Us
- The fully synthetic vaccine we provide has more B-cell display sites and an efficient immune response.
- The immunological results of the modified vaccine indicated higher antibody titers cross-reacting with the native Tn antigen.
- Antigen-modification strategies we provide can be widely used in the development of carbohydrate vaccines and other types of vaccines.
CD BioGlyco provides high-quality carbohydrate click-based sugar-conjugated vaccine development service to our clients and accelerates the process of GlycoCLICK™-based vaccine discovery and development through efficient and accurate strategies. Please feel free to contact us.
References
- Song, C.; et al. Synthesis and immunological evaluation of N-acyl modified Tn analogues as anticancer vaccine candidates. Bioorg Med Chem. 2016, 24(4): 915-20.
- Sletmoen, M.; et al. Tn and STn are members of a family of carbohydrate tumor antigens that possess carbohydrate-carbohydrate interactions. Glycobiology. 2018, 28(7): 437-442.
- Pifferi, C.; et al. Chemical synthesis and immunological evaluation of new generation multivalent anticancer vaccines based on a Tn antigen analogue. Chem Sci. 2020, 11(17): 4488-4498.
For research use only. Not intended for any clinical use.
Related Services
