Ganglioside Antigen Vaccine Development Service
Ganglioside Antigen: Effective Targets for Immunotherapy
CD BioGlyco provides various GlycoCLICK™-based Vaccine Development services such as Triazole Skeleton-based Vaccine, Carbohydrate Click-based Sugar-conjugated Vaccine, and Multivalent Glycopeptide-lipopeptide Vaccine development service. Ganglioside is a glycolipid with an oligosaccharide head group of one or more salivary acids attached to a ceramide. Its negatively charged head group extends out of the extracellular side of the plasma membrane and plays a key role in intercellular recognition. The major gangliosides expressed in the nervous system and cancer include GD3, GD2, and GM2. They bind specifically to their respective antigens with high affinity and display potent immune effector functions. Based on this feature, CD BioGlyco offers a highly tumor-selective ganglioside antigen vaccine development service.
Your Trusted Ganglioside Antigen Vaccine Development Partner
- Synthesis and Assembly Strategy
First, we construct functionalized galactose modules as linkers through a Cu-catalyzed (click) triazole formation reaction. At the same time, we provide site-specific modification services. All modules are attached to the alkyne linker via amide bonds, paving the way for "click" sialylation. Our researchers provide orthogonal analysis of the size and structural composition of all coupled and assembled, functionalized constructs. For the synthetic constructs, we provide high-performance liquid chromatography (HPLC) purification and yield testing services.
- Ganglioside Antigen Conjugation Service
We provide ganglioside antigen conjugation glycolipids (e.g. α-galactosylceramide) service, which enhances the immune response to co-delivered cancer antigens.
- Immunological Evaluation
Our research group evaluates the safety, immunogenicity, and toxicity of ganglioside antigen vaccine in animal models such as mice. We use single-dose and two-dose immunization to evaluate the immune response and cross-protection against viruses in mice. Depending on the needs of our clients, we provide model construction services and immunization programs. Our researchers optimize the immunization dose, immunization interval, and immunization sequence to ensure the best service for our clients.
Publication
Technology: Combinatorial chemistry, Weak affinity chromatography
Journal: Chemistry-A European Journal
Published: 2010
IF: 4.3
Results: In this study, we synthesized a library of GM1 ganglioside nonhydrolyzable mimics by click chemistry. The terminal alkyne and salivary acid azide of the linker were utilized in a copper-catalyzed (click) triazole formation reaction using the triazole to attach NeuAc residues to the linker. Galactose analogs of the linker arms were obtained by coupling different Gal fragments to variable-length linear alkyne linkers via amide bonds. Notably, the addition of salivary acid residues using click chemistry was the last and most important step. The affinity of gangliosides for cholera toxin was evaluated after weak affinity chromatography and found to be much higher than the affinity of individual pharmacodynamic sugar residues. Thus, the click chemistry-based linkage method developed in this study can be used as a rational platform for ligand design and development.
Fig.1 General strategy for library assembly. (Cheshev, et al., 2010)
Applications
- Ganglioside antigen vaccines modulate selective immune responses against cancer cells.
- Ganglioside antigen vaccines are important for the development of vaccines and monoclonal antibodies that target carbohydrate antigens.
- Ganglioside antigen vaccines promote the interaction of immune cell populations produce highly effective adaptive immunity, and are very promising anticancer vaccines.
Advantages of Us
- The modular synthesis strategy we provide is a viable pathway for a specific synthetic vaccine and is a key tool for analyzing the activation of the immune response in various biomedical applications.
- To accelerate the Huisgen azide-alkyne 1,3-dipolar cycloaddition (CuAAC) reaction rate and improve the conversion rate, our professional synthetic personnel multifactor optimization reaction includes but is not limited to catalyst excess, multiple Cu (I), ligand ratio, different reducing agent concentration, etc.
- The synthetic strategy we offer based on these junctional chemicals lays the stage for the design of novel synthetic vaccines of similar construction.
CD BioGlyco provides a multi-functional step-by-step click route, combined with the CuAAC connection, to efficiently develop the ganglioside antigen vaccine. Please feel free to contact us.
References
- Cheshev, P.; et al. Synthesis and affinity evaluation of a small library of bidentate cholera toxin ligands: towards nonhydrolyzable ganglioside mimics. Chemistry-A European Journal. 2010, 16(6): 1951-1967.
- Romanò, C.; et al. Chemical synthesis and immunological evaluation of cancer vaccines based on ganglioside antigens and α-Galactosylceramid. 2021.
For research use only. Not intended for any clinical use.
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