Azide (Az)/Dibenzylcyclooctyne (DBCO)-based Click Imaging Service
Unlocking Boundless Potential with Azide (Az)/Dibenzylcyclooctyne (DBCO)-based Click Imaging
Click labeling technique based on Az and DBCO is a very effective biomolecular labeling method. This technique can be used to study the localization, interaction, and metabolic pathways of biomolecules in cells, which is important for a deeper understanding of life sciences. Drawing on extensive experience in delivering GlycoCLICK™-based Imaging Service, CD BioGlyco presents clients with tailored Az/Difluorocyclooctene (DIFO)-based Click Imaging Services, Trans-cyclooctene (TCO)/Tetrazine (Tt) Ring-based Click Imaging Services and Az/DBCO-based click imaging services. Our Az/DBCO-based click imaging services are outlined below.
- We employ Az and DBCO click reactions for site-specific conjugation of fluorescent probes or other markers to biomolecules facilitating visualization of these biomolecules within cells or tissues.
- We offer specific molecule labeling services inside or on the surface of living cells through Az and DBCO click reactions, to track and localize dynamic changes in cellular structures or molecules in real-time.
- Our experts utilize Az/DBCO click fluorescent labeling, enabling high-resolution imaging and quantitative analysis of specific molecules in biological samples, helping you to further investigate biological processes and related mechanisms.
- CD BioGlyco utilizes Az and DBCO click reactions for protein cross-linking modifications to help you study protein interactions, complex formation, and other biological processes. We achieve real-time monitoring and visualization of protein interactions and complex formation by cross-linking proteins for modification with Az/DBCO click reaction and introducing fluorescent markers or other imaging probes.
Fig.1 Az/DBCO-based click imaging service. (CD BioGlyco)
Publication Data
Technology: Metabolic labeling
Journal: Angewandte Chemie International Edition
IF: 16.6
Published: 2016
Results: The main focus of this paper is on the use of Az and DBCO for targeted ultrasound-assisted selective chemo-labeling of cancer and subsequent cancer imaging. The researchers used an Az-containing glycan (Ac4ManAz) and a DBCO-containing fluorescent dye (DBCO-Cy5), which were released from microvesicles by ultrasound and metabolically expressed in the tumor region. The released Ac4ManAz was able to label Az moieties on tumor cells and significantly increased the accumulation of DBCO-Cy5 in tumors through subsequent click chemistry. This study demonstrates for the first time that Ac4ManAz-loaded microbubbles coupled with targeted ultrasound can be a simple and powerful tool for in vivo cancer-selective labeling and targeted cancer research.
Fig.2 Metabolic labeling of Ac4ManAz followed by DBCO-cargo targeting. (Wang, et al., 2016)
Applications
- Az/DBCO-based click imaging can be used for the labeling of biomolecules such as proteins, nucleic acids, and cell membrane components for visualization or quantitative analysis in cells or tissues.
- The combination of fluorescent probes by click reaction enables highly sensitive and specific cell or tissue imaging, which can be used to observe biological processes and disease development.
- The click imaging technique can be used to design and evaluate Az- and DBCO-modified drug delivery systems to improve the efficiency of targeted drug delivery and intracellular release.
Advantages
- The Az-based and DBCO compounds are biocompatible in living organisms and do not cause toxicity or side effects on biological systems, making them suitable for live cell imaging and in vivo studies.
- Our Az/DBCO-based click imaging can generally be carried out at room temperature, the reaction conditions are mild and do not require high temperatures or special environments, which is favorable for the processing and application of biological samples.
- The compounds containing an Az group exhibit structural diversity and can be synthesized and modified to meet different experimental needs, providing more options and flexibility.
Frequently Asked Questions
- What are the characteristics of Az/DBCO-based click imaging techniques for intracellular applications?
Az/DBCO-based click imaging technology is suitable for labeling a variety of biomolecules, such as proteins, DNA, RNA, etc., to study the functions and interactions of different biomolecules in cells. Meanwhile, Az/DBCO-based click imaging can be used for fluorescence imaging, two-photon imaging, in situ hybridization, and other imaging modes, which can realize high-resolution imaging of different types of biomolecules. Because of the advantages of this technology, such as fast speed and high specificity, biomolecules can be labeled and tracked during their dynamic processes, thus revealing their physiological functions and pathological mechanisms in the cell.
- How to choose the right Az/DBCO markers and fluorescent probes for cell imaging experiments?
Depending on the biomolecule you intend to label (e.g. protein, nucleic acid, etc.), we carefully select a marker possessing the corresponding Az/DBCO functional group. It is crucial to ensure that the chosen marker exhibits high specificity and affinity towards Az/DBCO to prevent any non-specific binding or background signal interference. If labeling within living cells is necessary, it is advisable to opt for a marker with excellent cell permeability to guarantee optimal effectiveness and stability during the labeling process.
CD BioGlyco offers Az/DBCO-based click imaging service, providing clients with efficient, specific, and biocompatible biomolecular labeling solutions. Our advanced click labeling technology enables a wide range of applications including fluorescence imaging, cell tracking, protein cross-linking studies, and more. If you are interested in our services, please contact us for any further assistance.
Reference
- Wang, H.; et al. Targeted ultrasound‐assisted cancer‐selective chemical labeling and subsequent cancer imaging using click chemistry. Angewandte Chemie International Edition. 2016, 55(18): 5452-5456.
For research use only. Not intended for any clinical use.
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