CD BioGlyco has dedicated extensive efforts to refining the GlycoCLICK™ technology over the past few years. This technology offers a distinctive method for visualizing biomolecules. By leveraging click chemistry, our GlycoCLICK™-based imaging services facilitate the selective attachment of labels to target molecules, enabling precise visualization and imaging of specific biomolecules. Our GlycoCLICK™-based imaging services are subdivided into two categories based on structure and type:

By Structure

GlycoCLICK™-based Triazole Skeleton Molecule Imaging Service

CD BioGlyco offers our clients a customized GlycoCLICK™-based triazole backbone molecule imaging service. These include our professional covalently labeled radionuclide imaging service and metal chelates-based radioactive tracers imaging service, which have been well received by clients.

GlycoCLICK™-based Oxime Skeleton Molecule Imaging Service

In the field of bioimaging, oxime skeleton molecules are widely used for labeling and imaging of biomolecules and cells. CD BioGlyco offers clients a customized GlycoCLICK™-based oxime skeleton molecule imaging service.

GlycoCLICK™-based Disulfide Bonded Molecule Imaging Service

Disulfide bonds are commonly found in biomolecules such as proteins, enzymes, and antibodies. CD BioGlyco offers our clients the GlycoCLICK™-based disulfide bonded molecule imaging service, which helps clients to explore the role of disulfide bonds in protein structure and function, and disease development.

By Type

Azide (Az)/Difluorocyclooctene (DIFO)-based Click Imaging Service

CD BioGlyco provide Az/DIFO-based click imaging service, which employs the chemical reaction of Az and DIFO to attach them onto biomolecules. It enabling the visualization and quantification of the spatial distribution within cells or tissues.

Az/Dibenzylcyclooctyne (DBCO)-based Click Imaging Service

We usually introduce Az functional groups into the target molecule. Then, a fluorescent/ radionuclide probe with DBCO functional groups is added to the sample to be imaged. When the Az and DBCO functional groups come into contact with each other and binds specifically to the target molecule.

Trans-cyclooctene (TCO)/Tetrazine (Tz) Ring-based Click Imaging Service

TCO and Tz rings form stable covalent linkages by click reactions in the presence of suitable reducing agents. We offer the TCO/Tz ring-based click imaging service which utilizes double peptide bond redox click reactions for molecular labeling and imaging.

The imaging techniques we utilize in our GlycoCLICK™-based imaging service include but are not limited to:

Fluorescent probes	Fluorescent probes are fluorescent labeling agents that bind specifically to biomolecules and emit a fluorescent signal. We achieve highly sensitive imaging and quantitative analysis of biomolecules by using fluorescent probes in combination with click reactions.
Optical microscope	When imaging fluorescent probes, the optical microscope is an important imaging tool. A light microscope is a high-resolution imaging tool that can be used to image biological samples at the cellular and molecular levels. By adjusting the imaging parameters of the optical microscope, high-quality fluorescence imaging data can be obtained.
Computer processing and image analysis	We process, analyze, and visualize imaging data through the use of computer-aided analysis tools.

Fig.1 Flowchart of GlycoCLICK™-based triazole skeleton molecule imaging service. (CD BioGlyco)

Publication Data

Technology: Radioimaging

Journal: Coordination Chemistry Reviews

IF: 24.833

Published: 2017

Results: This article discusses the applications of chelating agents for radioactive lanthanides in imaging and therapy. Applications of chelators of radioactive lanthanides for imaging include the use of chelators such as DOTATATE and DOTANOC bound to radioactive lanthanides for positron emission tomography (PET) imaging and single photon emission computed tomography (SPECT) imaging. In particular, DOTATATE combined with 177Lu is used for PET imaging, while DOTANOC combined with 177Lu is used for PET imaging of neuroendocrine tumors. In addition, several other chelators bind to radioactive lanthanides for imaging and treating other types of tumors. Therapeutic applications of chelators of radioactive lanthanides include the use of chelators such as DOTATATE and DOTANOC in combination with radioactive lanthanides for the radiotherapy of tumors. For example, DOTATATE is used in combination with 177Lu for the treatment and diagnosis of cancer. In addition, several other chelating agents are combined with radioactive lanthanides for the treatment of other types of tumors. The article also provides information on the potential use of these chelators in radiographic imaging and radiotherapy.

Fig.2 Bio-orthogonal click reactions commonly used for in vivo biological labeling and imaging applications. (Liu, 2019) Fig.2 Bio-orthogonal click reactions commonly used for in vivo biological labeling and imaging applications. (Liu, 2019)

Applications

GlycoCLICK™-based imaging enables the exploration of tumor marker detection and localization, facilitating effective visualization and localization within living cells and tissues.
GlycoCLICK™-based imaging can be applied to assist in detecting the presence, location, and size of tumors.
GlycoCLICK™-based imaging can be used to investigate glycosylation modifications during disease onset and progression, and the relationship between glycosylation modifications and disease.

Advantages

GlycoCLICK™-based imaging enables highly sensitive detection of glycosylation modifications at low concentrations.
GlycoCLICK™-based imaging technology can be used in combination with a wide range of imaging agents (e.g., fluorescent dyes, radioisotopes, etc.) to meet the diversity of different research needs.
The versatility of GlycoCLICK™-based imaging technology makes it highly applicable to numerous areas of research, including oncology, biology, and medical studies, making it an ideal addition to your research toolbox.

CD BioGlyco is a proficient provider of GlycoCLICK™-based imaging services, offering expertise and support at every stage of the imaging process to meet your specific needs. If you have any questions about our services, please feel free to contact us for more detailed information.

References

Liu, B. Bio-orthogonal click chemistry for in vivo bioimaging. Trends in Chemistry. 2019, 1(8): 763-778.
Amoroso, A.J.; et al. Chelating agents for radiolanthanides: applications to imaging and therapy. Coordination Chemistry Reviews. 2017, 340: 198-219.

For research use only. Not intended for any clinical use.

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