Modified Your Proteins with GlycoCLICK™ Innovation

Click chemistry is commonly used in protein modification, which enables efficient, rapid, and controllable protein modification. Commonly used click chemistry methods include alkyne-azidation and alkyne-olefin cycloaddition reactions. In protein modification, alkyne or olefinic groups are usually introduced on the surface of proteins, and then click reactions are carried out with the corresponding azide reagents or olefinic reagents to achieve protein modification. CD BioGlyco provides an all-inclusive selection of services that assist our clients in their research. Our team of experts and product managers are accessible globally, offering guidance on GlycoCLICK™-based Modification Service, and are prepared to deliver dependable GlycoCLICK™-based protein modification services. The details of our GlycoCLICK™-based protein modification services are as follows:

• First, we synthesize unnatural amino acids with alkyne groups by chemical synthesis. These unnatural amino acids contain alkyne groups, azide groups, and other functional groups that are necessary to click reactions and can be selectively captured by another compound containing electrophilic reactive functional groups.
• Then, we introduce genes for unnatural amino acids containing alkynyl groups into host cells to express the target proteins in the cells. Protein expression is promoted by adding appropriate inducers or adjusting conditions such as temperature and pH. The target proteins are collected from the cells and undergo a series of purification steps to obtain highly purified target proteins.
• Finally, we add compounds containing azide groups to the purified target proteins obtained by click reaction with unnatural amino acids containing alkyne groups. The modified proteins are then analyzed using mass spectrometry and electrophoresis to determine the location and extent of modification.

Publication Data

Applications

• By linking markers such as fluorescent dyes, biotin, etc., it can be used for localization and visualization of proteins.
• By linking small molecules with specific functions, such as drugs, polymers, etc., it is applied to change the properties and functions of proteins.
• By linking two proteins or covalent modifiers of proteins and other molecules, protein complexes with specific functions can be constructed.

Advantages

• GlycoCLICK™-based protein modification is carried out under mild conditions and does not significantly affect the structure and function of proteins.
• GlycoCLICK™-based protein modification can be applied to a wide range of different functional groups, enabling diverse protein modifications.
• The click reaction usually exhibits high reaction selectivity and high reaction efficiency. This means that specific protein modifications can be achieved in complex biological systems and the modification reaction can be completed in a short time.

CD BioGlyco offers a one-stop GlycoCLICK™-based protein modification solution for your protein modification research project, ensuring success and minimizing challenges at every stage. Our team of experts will guide and support you throughout your project, guaranteeing its success in various aspects. For further details, please don't hesitate to contact us.

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