Fast-on-the-clock Delivery Multivalent Glycopeptide-lipopeptide Vaccine Development Solutions

With the advanced GlycoCLICK™-based Vaccine Development platform, CD BioGlyco efficiently combines glycopeptides with multivalent lipopeptides to provide reliable vaccine development services for our clients. We provide a comprehensive one-stop service covering antigen discovery, solid phase synthesis, animal model building, and vaccine assessment. We tailor solutions to meet the special needs of our clients. Moreover, we also provide reliable Triazole Skeleton-based Vaccine and Carbohydrate Click-based Sugar-conjugated Vaccine Development services.

• Synthetic Service

We provide synthetic services for a variety of substances including the synthesis of solid-phase glycopeptides, the synthesis of lipopeptide Pam3CysSer-(Lys)4-OH (Pam3CSK4), the synthesis of lipopeptides with functionalized toll-like receptor (TLR) ligands such as with mono-, di-, and tetravalent. All the compounds are purified multiple times to ensure that the impurities do not affect subsequent reactions.

• Copper(I)-mediated Click Reaction

After high-performance liquid chromatography (HPLC) purification, we couple the glycopeptides to the functionalized lipopeptides by click chemistry to obtain a multivalent glycopeptide-lipopeptide vaccine. We covalently couple antigens such as glycopeptides with the carrier proteins to enhance the immune response. Moreover, we provide accurate molecular weight determination services for synthetic vaccines with electrospray ionization quadrupole-time-of-flight mass spectrometry (ESI-QTOF-MS).

• Immune Assessment Service

We use flow cytometry to detect the binding of antisera to antigens in cells. Meanwhile, the survival rate of tumor cells is determined by brominated 3-(4,5-dimtheylthiazol-2-yl)-2,5-diphenltetrazoliumbromide (MTT) assay, and the mortality rate is recorded by lactate dehydrogenase (LDH) assay. Moreover, we provide antibody potency analysis services.

Our research team provides animal models for assessing the immune responses elicited by synthetic vaccines. Culture for some time by intraperitoneal administration and immunization for specific periods. We use an enzyme-linked immunosorbent assay (ELISA) to detect the total induced antibodies.

Publication

Applications of Multivalent Glycopeptide-lipopeptide Vaccine

• Multivalent glycopeptide-lipopeptide vaccine couples with a tetanus toxoid to obtain a high-quality antitumor vaccine.
• Multivalent glycopeptide-lipopeptide vaccine development is an effective target for cancer therapy.
• The multivalent glycopeptide-lipopeptide vaccine is important for improving immunogenicity.

Advantages

• We use ESI-QTOF-MS to measure molecular weight because it has higher accuracy, especially the detection of macromolecules is also very high.
• Vaccines developed to combine glycopeptides with lipopeptides have fewer side effects and apply to humans.
• Multivalent glycopeptide-lipopeptide vaccine triggers an immune response in mice without any external adjuvant.

CD BioGlyco provides professional GlycoCLICK™-based vaccine development service. We have an efficient synthesis team and experienced staff to provide perfect service to our clients. For more information about the multivalent glycopeptide-lipopeptide vaccine development service, please feel free to contact us.

• Triazole Skeleton-based Vaccine Development Service
• Carbohydrate Click-based Sugar-conjugated Vaccine Development Service