Your Trusted Partner for Azide-based Cell Surface Glycan Labeling

Metabolic glycan engineering is a technique that uses monosaccharide analogs to install unnatural sugars into the glycocalyx of mammalian cells and has recently been used to label and target cancer cells. In this technique, cells are incubated with monosaccharide analogs bearing unnatural chemical groups (e.g., azides, thiols, ketones, etc.), and these monosaccharides are processed by biosynthetic enzymes in a manner analogous to their natural precursors, resulting in cell surface glycans bearing unnatural chemical groups. At CD BioGlyco, our GlycoCLICK™-based Labeling Service has been consistently well-received by our clients. Our azide-based GlycoCLICK™-based Cell Surface Glycan Labeling Service enables glycan labeling by combining our GlycoCLICK™ technology with metabolic glycan engineering. The azide-modified glycan molecules or fluorescent probes are used to react directly with cell surface glycan structures to achieve selective labeling and detection of specific glycan structures. The azide-based cell surface glycan labeling services we provide include but are not limited to:

• N-Azidoacetylneuraminic acid (Neu5Az)-based cell surface glycan labeling service
• N-Azidoacetylgalactosamine (GalNAz)-based cell surface glycan labeling service
• N-Azidoacetyl-D-mannosamine (ManNAz)-based cell surface glycan labeling service
• N-Azidoacetylglucosamine (GlcAz)-based cell surface glycan labeling service
• 6-Azido fucose (FucAz)-based cell surface glycan labeling service
• N-Azidoacetyl sialic acid (SiaNAz)-based cell surface glycan labeling service

By introducing these glycans into the cell, we effectively introduce azides into the cell surface glycan structure. The introduced azide reacts with specific reagents to achieve selective labeling of cell surface glycans. After the reaction, the labeled cell surface glycan structure shows fluorescence or signal enhancement. This enables the labeled cells to be visualized and analyzed by imaging techniques such as fluorescence microscopy, thus helping your research on the distribution, composition, and dynamics of the glycans.

Publication Data

Applications

• This technique allows for the selective detection and imaging of cell surface glycans by introducing fluorescent or chemical probes with specific azide groups. Therefore, it is utilized to uncover the composition, distribution, and dynamics of cell surface glycans accurately.
• This technique is used to detect and analyze glycoprotein interactions by reacting azide-modified glycan molecules or glycoproteins with target proteins.
• This technique achieves selective recognition and labeling of specific cell types by binding azide-modified sugar molecules to specific sugar receptors on the cell surface, which can be used for cell identification and classification.

Advantages

• Azide-based cell surface glycan labeling allows precise labeling and detection of the target glycan structure and avoids non-specific background signal interference.
• Azide-modified fluorescent probes or chemical probes with high sensitivity enable rapid, accurate, and real-time detection of cell surface glycans.
• This technique visualizes and reveals the spatial distribution and dynamics of cell surface sugars, providing intuitive information for functional studies of cells and tissues.

CD BioGlyco is a company dedicated to the research and application of glycans. Our team of experts collaborates with clients to develop optimal experimental protocols based on experimental needs and target cell types to offer azide-based cell surface glycan labeling services. Please feel free to contact us if you have any inquiries regarding our services.

• Alkynyl-based Cell Surface Glycan Labeling Service