Efficient Click Chemistry Strategies for Complex O-Glycan Cluster Synthesis at CD BioGlyco

Click chemistry reactions are often highly selective and achieve specific ligation in complex mixtures, which is very critical for the synthesis of O-glycan clusters, because the sugar chain structure is complex and diverse, and specific sugar units need to be connected accurately. Based on Click Chemistry Technology, CD BioGlyco provides high-level O-glycan cluster synthesis services.

• Design synthetic route: Based on the final O-glycan cluster structure and its modification pattern, our scientists select the appropriate core structure, connection method, and modification group to design a reliable synthetic route.
• Synthesis of sugar chain precursors: According to the design plan, we synthesize the precursors of the O-glycan cluster, which are usually sugar chain molecules with simplified structures. This process is achieved through chemical synthesis, enzyme catalysis, and other methods. Sugar chain precursors are usually modified to contain functional groups required for click reactions, such as alkynyl or azide groups.
• Click reaction: We perform a click reaction between the synthetic precursor and a suitable click reagent, which is usually carried out under appropriate solvents and conditions, and requires a suitable catalyst to promote the reaction process. We usually use alkynyl-modified sugar chain precursors and derivatives containing corresponding azide groups to perform continuous click reactions to synthesize O-glycan clusters containing triazole rings. Click chemical reactions are generally carried out under mild conditions and do not require the use of strong acid, strong alkali, or high-temperature conditions, thus helping to protect sensitive sugar chain structures and avoid their degradation or damage.
• Purification and analysis: We purify the synthesized products to remove unreacted materials. Moreover, the purified products are structurally identified and quantitatively analyzed using mass spectrometry, nuclear magnetic resonance, and other technologies.
• Large-scale synthesis: We also provide large-scale O-glycan cluster synthesis and production services to obtain sufficient amounts of O-glycan cluster products.

O-Glycan Cluster Modification and Structure Analysis Service at CD BioGlyco

• During the synthesis process of the O-glycan cluster, we also use click chemistry technology to provide O-glycan cluster modification services. At the same time, the modified sugar chains are verified through mass spectrometry (such as time-of-flight mass spectrometry, electrospray mass spectrometry, etc.) and chromatographic analysis to determine the position and structure of the modification.
• For synthesized or modified O-glycan clusters, we provide structural analysis services through nuclear magnetic resonance and other analysis methods (such as X-ray crystallography, two-dimensional nuclear magnetic resonance spectroscopy, etc.). Detailed information on the modified linkage pattern, sugar unit sequence, and stereoconfiguration are thus determined.

In addition, CD BioGlyco has extensive experience in the field of GlycoCLICK™-based Glycocluster Synthesis. Our scientists are familiar with the synthesis methods, technologies, and processes of the glycocluster. In addition to the synthesis of O-glycans, we also provide C-Glycan, S-Glycan, and Heteropolysaccharide Cluster synthesis services.

Publication

Applications

• Cancer research: The click chemistry-based O-glycan cluster synthesis method is used to study O-glycosylation modifications in tumor markers and explore its relationship with cancer development and progression, which helps develop new tumor diagnostic markers.
• Medical imaging: Introducing fluorescent markers or contrast agents containing O-glycan clusters into medical imaging is used for biomarker detection and tumor imaging, which helps improve early detection and localization of cancer.
• Drug screening: O-glycan cluster synthesis is used for drug development and screening, especially for diseases related to glycosylation modifications. Using click chemistry technology to synthesize compounds with different O-glycan clusters, their interactions with sugar receptors are evaluated and new drug candidates are found.

Advantages

• CD BioGlyco has extensive experience in the field of glycocluster synthesis, and we provide various synthesis services according to client requirements.
• CD BioGlyco provides synthesis for different types of O-glycan clusters, including the synthesis of different sugar groups, connection methods, and modification patterns.
• CD BioGlyco provides high-quality O-glycan cluster analysis to ensure the quality and purity of synthesized products.

CD BioGlyco provides high-level O-glycan cluster synthesis services. We design and optimize synthesis strategies to improve synthesis efficiency and product purity and ensure reliable results and delivery. Please feel free to contact us if you would like to inquire for more details.

• C-Glycan Cluster Synthesis Service
• S-Glycan Cluster Synthesis Service
• Heteropolysaccharide Cluster Synthesis Service