GlycoCLICK™-based Vaccine Development Service
GlycoCLICK™-based Vaccine Development Service at CD BioGlyco
Many pathogens (such as bacteria, viruses, and parasites) and some tumor cells have specific sugar molecules on their surfaces. These sugar molecules serve as markers or antigens for pathogens and participate in their infection, invasion, immune evasion, and other processes. Sugar molecules therefore serve as important targets for vaccine development. CD BioGlyco provides specialized GlycoCLICK™-based vaccine development service.
Target determination service
We first select the pathogen or disease that needs to be targeted, and by studying the glycobiological characteristics of the target pathogen, we determine the specific sugar structure as a target for vaccine development. These sugar structures are usually located on the surface of pathogens and play key roles in adhesion and entry into host cells.
Glycosyl synthesis service
We synthesize the required sugar molecules through a variety of synthetic pathways, such as chemical synthesis, enzymatic reactions, and biosynthesis. Then we use click chemistry technology to introduce specific reactive groups on the synthesized sugar molecules, such as alkenes, alkynes, etc., which will be used in subsequent click reactions.
Carrier protein preparation service
We select suitable carrier proteins, such as peptides, protein fragments, or full-length proteins, as scaffolds for glycoconjugated vaccines. These carrier proteins generally have good immunogenicity and stability.
Sugar-carrier complexes preparation service
We perform a click reaction between the synthesized glycosyl material and the carrier protein to form a covalently linked sugar-carrier complex. This click reaction usually utilizes a cyclization reaction between a fused iodonium salt and an azoalcohol to stably link the glycosyl moiety to the carrier protein. CD BioGlyco provides many types of GlycoCLICK™-based vaccine development services.
Vaccine optimization and modification service
Click chemistry technology is applied to different types of sugar molecules and protein carriers, making vaccine development more flexible and diverse. By adjusting reaction conditions and designing appropriate click reaction sites, we achieve targeted modifications to different types of vaccines, such as introducing immune adjuvants, thereby improving the immunogenicity and efficacy of the vaccine.
Vaccine assessment service
We evaluate the immunogenicity of vaccines through in vitro experiments (such as ELISA) and animal experiments. These experiments determine whether the vaccine induces specific antibodies against the target sugar structure.
Vaccine production service
Click chemistry technology has good scalability and reproducibility, and we use click chemistry technology to provide large-scale production for sugar-conjugated vaccines.
Fig.1 GlycoCLICK™-based vaccine development service. (CD BioGlyco)
Publication
Technology: Click chemistry
Journal: ACS Omega
IF: 4.132
Published: 2022
Results: The authors used click chemistry to couple a cell-free protein synthesis (CFPS) platform, and used this platform to precisely conjugate group A carbohydrates (GAC) with C-terminal truncated streptolysin O (SLO) carrier protein. An immunogen was produced that produced functional antibodies against group A Streptococcus (GAS) virulence factors. This result suggested that click chemistry helped speed up the process of vaccine development.
Fig.2 Click chemistry-based vaccine development process. (Kapoor, et al., 2022)
Applications
- Cancer immunotherapy research: Click chemistry techniques are applied to synthesize specific glycosylated antigens and incorporate them into immune cell vaccines. These glycosylated antigens enhance the immune system's recognition and attack of cancer cells, thus improving the effectiveness of cancer immunotherapy.
- Modulating immune responses: Click chemistry techniques are used to modulate the strength and type of immune responses. By synthesizing glycosylated vaccines with different structures, different cell types in the immune system are selectively activated, thereby regulating specific immune responses.
- Personalized vaccines: Click chemistry technology enables highly personalized vaccine development. By synthesizing specific glycosylation structures, personalized vaccines are designed and prepared according to individual immune genotypes and disease characteristics.
Advantages
- Leading technology: CD BioGlyco has advanced click chemistry technology and professional vaccine development teams. We apply the latest scientific research results to conduct multi-angle and comprehensive vaccine research and development.
- Personalized design: CD BioGlyco uses click chemistry technology to provide personalized vaccine design and development services according to the characteristics of different pathogens and diseases.
- Efficiency: Click chemistry technology has fast and efficient reaction speed and directionality of reaction products, which allows us to respond faster to the threats of emerging diseases and provide faster vaccine solutions, thus accelerating the process of vaccine development.
CD BioGlyco utilizes click chemistry technology to provide various types of glycosylated vaccine development services, including polysaccharide, protein, and glycan-conjugated vaccines. Through click chemistry technology, we precisely control the location, amount, and distribution of glycosylation to ensure that the synthesized vaccine has the desired structure and function. Please feel free to contact us if you would like to acquire details on the development of the vaccine.
Reference
- Kapoor, N.; et al. Non-native amino acid click chemistry-based technology for site-specific polysaccharide conjugation to a bacterial protein serving as both carrier and vaccine antigen. ACS Omega. 2022, 7(28): 24111-24120.
For research use only. Not intended for any clinical use.
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