GlycoCLICK™-based Ribose Modification Service

Unlocking Potential with GlycoCLICK™-based Ribose Modification

Ribose modifications based on the click reaction are used for a variety of applications. One of the most common applications is the use of click reactions to introduce some specific structures into the ribose backbone for operations such as labeling, ligation, and cyclization. At CD BioGlyco, the click reaction that we commonly use in ribose modification is azide-alkyne cycloaddition (AAC). Through this, alkyne or azide groups can be introduced into the ribose backbone for labeling and ligation of nucleic acids. The GlycoCLICK™-based ribose modification service we provide includes but is not limited to the following:

Azide-based Ribose Modification Service

Azido-based ribose modification is a versatile and highly selective method that involves the cyclization of an azide group with an alkyne group on a ribose to form a stable cyclic compound. Our azide-based ribose modification service allows for highly selective modification of specific RNA molecules. Specific sites of the RNA molecule are precisely targeted and modified by strategically introducing azide groups at desired locations on the ribose backbone without affecting the rest of the molecule.

Alkynyl-based Ribose Modification Service

Our alkynyl-based ribose modification service is compatible with a wide range of functional groups, making it highly versatile for a variety of applications. We use a variety of azide and alkynyl derivatives to introduce different functionalities on the RNA molecule depending on your needs, including fluorescent probes, affinity labels or chemical treatments for further conjugation or detection.

GlycoCLICK™-based ribose modification service. (CD BioGlyco)

Publication Data

Technology: Nucleic acid labeling and nucleic acid construction

Journal: Chemical Reviews

IF: 72.087

Published: 2021

Results: This article describes applications and guidelines for nucleic acid click chemistry. The article discusses the application of click chemistry to nucleic acid modification, labeling, and synthesis. Click chemistry is a highly efficient, specific, and reversible chemical reaction that can be used to introduce a variety of functional groups into nucleic acid molecules, such as fluorescent dyes, biologically active molecules, and immobilization groups. The article also provides guidelines on selecting appropriate click reactions, reaction conditions, and reactants, as well as how to design and synthesize nucleic acid probes and hybrids required for click chemistry reactions. The article mentions that click chemistry modification methods have several applications in RNA modification. Among them, click chemistry can label other small organic compounds onto oligonucleotides to increase the uptake of nucleic acids into cells. Click chemistry can also directionally target reactive inorganic compounds to specific genomic sequences. In addition, the methods of click chemistry modification have been investigated for the assembly of nucleic acids for applications in biology and nanotechnology. Through the use of pure synthetic chemistry, long sequences of nucleic acids can be assembled by efficiently joining DNA strands using the base-recognition capabilities provided by nucleic acids.

Fig.1 Examples of alterations to nucleotides to introduce alkyne into oligonucleotides. Fig.1 Examples of nucleosides that have been altered to introduce alkyne functionalities into oligonucleotides. (Fantoni, et al., 2021)

Applications

GlycoCLICK™-based ribose modification has been used to introduce base modifications and nucleotide derivatives (e.g., locked nucleotides, G-clamp) to antisense oligonucleotides.
Fluorescent dyes are labeled onto oligonucleotides by using GlycoCLICK™-based ribose modification technology for use in fields such as DNA sequencing, genomics, and forensic science.
The application of GlycoCLICK™-based ribose modification in the field of clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) gene editing is investigated for the rapid high-throughput generation of single guide RNA libraries.

Advantages

GlycoCLICK™-based ribose modification is highly biocompatible in living organisms, and modification reactions can be performed in living organisms without causing significant toxicity or side effects.
GlycoCLICK™-based ribose modification enables target ribose can be selectively modified without affecting other molecular structures.
The GlycoCLICK™-based ribose modification is usually performed at room or lower temperatures, without the use of intense conditions such as high temperatures or strong acids and bases, which do not have a destructive effect on biological samples and are conducive to the maintenance of biological activity.

With more than a decade of experimental experience, CD BioGlyco aims to fully support clients' research by providing professional GlycoCLICK™-based ribose modification services according to their specific requirements. Please feel free to contact us to discuss your specific needs.

Reference

Fantoni, N.Z.; et al. A hitchhiker's guide to click-chemistry with nucleic acids. Chemical Reviews. 2021, 121(12): 7122-7154.

For research use only. Not intended for any clinical use.

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