Nanometabolic Precursors (Nano-MP)-based Drug Delivery Service

Experience the Exceptional Nanometabolic Precursors (Nano-MP)-based Drug Delivery Service

Nano-MP is a novel concept in the field of medicine and biotechnology, which refers to a nanoscale substance that can be converted into an active metabolite in the body for precise drug delivery and therapeutic effects. The concept of nano-MP is based on an understanding of the application of nanotechnology to drug delivery and biological effects. At CD BioGlyco, our team possesses extensive experience and expertise, accompanied by remarkable accomplishments in the realm of drug delivery. Our unwavering dedication lies in delivering exceptional GlycoCLICK™-based Drug Delivery Service. Notably, our nano-MP-based drug delivery service stands as a pivotal offering among our comprehensive range of services. The specifics of our service are outlined below.

To begin with, we synthesize azide moieties containing azide groups by chemically conjugating the metabolic precursor Ac₃ManNAz with nano-MPs composed of generation 4 poly(amidoamine) (PAMAM [G4]) dendrimer.
Nano-MPs possess high molecular weight and nanoscale size and can be localized in heterogeneous tumor cells through enhanced permeation and retention effects. Nano-MPs can be rapidly internalized by a majority of cancer cells and undergo hydrolysis in acidic conditions. This process leads to the production of azide-containing sialic acid derivatives, which can induce the formation of synthetic chemoreceptors on the surface of most tumor cells via the sialic acid biosynthesis pathway. These functional groups facilitate the creation of synthetic chemoreceptors on most tumor cell surfaces through activation of the sialic acid biosynthesis pathway.
Finally, we covalently bind bioorthogonal chemical groups labeled with a near-infrared fluorescent dye (Cy5.5) to an artificial chemoreceptor via an in vivo copper-free click chemistry reaction for targeted imaging of tumor cells.

Fig.1 Flowchart of nanometabolic precursors-based drug delivery service. Fig.1 Process of nano-MP-based drug delivery service. (CD BioGlyco)

Publication Data

Technology: In vivo/in vitro imaging, western blot, and flow cytometry

Journal: Biomaterials

IF: 7.59

Published: 2017

Results: This article discusses a novel tumor-targeting strategy that generates artificial chemoreceptors on the cell surface metabolic glycoengineering and irreversibly binds to bioorthogonal chemical moieties using copper-free click chemistry in vivo. This strategy could overcome the limitations of current single-molecule-based active targeting strategies in heterogeneous tumor cells from the same patient as well as across patients. Specifically, the researchers developed nano-MPs formed by binding a metabolic precursor containing an azo group to a polymer (PAMAM [G4] dendrimer) that can be metabolically glycoengineering to homogeneously generate azo groups on the cell surface of tumor cells regardless of cell type. These generated artificial chemoreceptors contain azo groups that can click chemically with bioorthogonal chemical groups. Under in vitro cell culture conditions, this strategy successfully generated artificial chemoreceptors with azo groups on the surface of heterogeneous tumor cells, whereas conventional receptors can only bind to specific tumor cells. In a mouse tumor model, it was localized to target tumor tissues mainly through enhanced permeation and retention effects and successfully generated azo groups on tumor cells after intravenous injection.

Fig.2 Graphical representation of nano-MP for targeting heterogeneous tumors using bioorthogonal click chemistry in living organisms. Fig.2 Graphical depiction of nano-MP to target diverse tumors through bioorthogonal click chemistry in vivo. (Lee, et al., 2017)

Applications

Nano-MP is applied to enable targeted drug delivery or molecular imaging of tumor cells by click chemistry, reacting with drug delivery vehicles or imaging probes labeled with bioorthogonal chemical molecules.
By modulating the rate of the click reaction, it can be used to control the rate and duration of drug release and to realize a long-acting drug delivery system.
Nano-MP can be used to improve drug targeting by being designed to recognize and bind to specific cells or tissues, such as tumor cells. This helps to minimize the impact on healthy cells and reduce side effects.

Advantages

Nano-MP is a nanoscale material with a large specific surface area and highly tunable properties, providing more space for effective drug delivery.
Nano-MP enables precise modulation of drug release rate and targeting by changing its shape, size, and surface properties.
Nano-MP is biocompatible and stable in vivo and does not cause significant toxic side effects or immune reactions. This enables nano-MP-based drug delivery systems to deliver drugs to target tissues or organs more safely and with less damage to healthy tissues.

CD BioGlyco places great emphasis on quality assurance throughout every step of the nano-MP-based drug delivery process. Our state-of-the-art facilities ensure accuracy and reproducibility in all experiments conducted. Please contact us, we welcome any inquiries regarding our services and products.

Reference

Lee, S.; et al. Nano-sized metabolic precursors for heterogeneous tumor-targeting strategy using bioorthogonal click chemistry in vivo. Biomaterials. 2017, 148: 1-15.

For research use only. Not intended for any clinical use.

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