GlycoCLICK™-based High-throughput Screening Service
GlycoCLICK™-based High-throughput Screening (HTS) Service at CD BioGlyco
HTS is a well-established process used for drug discovery in pharmaceutical and biotechnology companies and is now also used in basic and applied research in academia. It screens large chemical libraries for activity by using automated, miniaturized assays and large-scale data analysis to find compounds that are active against biological targets. Combining click chemistry with high-throughput enzyme assay technology has revolutionized primer discovery and optimization steps in drug discovery. CD BioGlyco is committed to combining click chemistry with powerful technologies to drive the rapid development of HTS and accelerate the drug discovery process, providing professional GlycoCLICK™-based HTS services to our clients.
Microarrays are high-throughput experimental platforms that allow the simultaneous testing of a large number of samples and reaction conditions. By applying click chemistry to compound synthesis on microarrays, large libraries of compounds can be rapidly generated and screened.
- First, we design and synthesize a series of compounds containing click chemistry reaction sites based on your needs. These compounds are structurally diverse. We then introduce appropriate functional groups such as alkynes or azides on the microarray surface to enable immobilization of the compound on the microarray. Then, we perform the reaction of the designed click compounds with the surface of the functionalized microarrays to form covalent connections and complete the microarray synthesis.
- We realize the loading of samples to be screened onto a microarray chip by droplet, osmotic, or other methods. The sample specifically binds or interacts with the probe molecules on the surface of the chip. After sample loading, we perform a washing step to remove non-specific binders and interferences.
- We analyze molecular interactions on the chip using appropriate detection methods. For example, we use fluorescent probes, mass spectrometry, and electrochemical methods to detect and quantify interaction signals. The obtained data are then analyzed and validated to assess the specific interactions of the sample with the probes on the microarray. This involves data processing, statistical analysis, and comparison of results between different samples.
Fig.1 Process of GlycoCLICK™-based HTS. (CD BioGlyco)
Publication Data
Technology: Bioorthogonal noncanonical amino acid tagging (BONCAT)
Journal: Chemical reviews
IF: 72.087
Published: 2013
Results: This article discusses the use of click chemistry in drug development and diverse chemical-biological applications. It provides information on the applications of click chemistry in drug development, including click chemistry in drug synthesis, drug screening, and drug delivery. The authors describe that in drug development, click chemistry is used to synthesize pharmacologically active heterocyclic molecules that can serve as potential drug precursors. Click chemistry can also be used to synthesize enzyme inhibitors, which can be generated by click chemistry reactions with high affinity and selectivity for the study and treatment of related diseases. In addition, click chemistry can be used to synthesize receptor ligands for receptor-ligand binding studies in drug development. Click chemistry can also be applied to innovations in biology, such as labeling proteins, glycans, lipids, and cell surfaces in biological systems through click chemistry reactions, enabling localization and functional studies of biomolecules.
Fig.2 Workflow of the process of hit-to-lead optimization from click chemistry and drug candidate selection. (Thirumurugan, et al., 2013)
Applications
- GlycoCLICK™-based HTS is applied for the screening of compound libraries to identify potential drug candidates with specific biological activities, such as enzyme inhibition, receptor binding, or modulation of cellular functions.
- GlycoCLICK™-based HTS is used to study protein-protein interactions, protein-ligand interactions, and other biomolecular interactions.
- It is employed in the discovery and development of new materials with desired properties, such as catalysts, polymers, and nanoparticles.
Advantages
- GlycoCLICK™-based HTS technology allows for rapid, automated screening of large numbers of samples, greatly improving screening efficiency.
- GlycoCLICK™-based HTS is suitable for different types of samples, including proteins, sugars, small molecules, etc., for all areas of your research.
- This technology detects trace amounts of glycosylation modification molecules with high sensitivity, which is important for studying the abundance and dynamics of glycosylation modifications.
CD BioGlyco offers a comprehensive GlycoCLICK™-based HTS service to support clients' research. Our team of product managers and experts is globally accessible, offering expert advice and guidance. Please feel free to feel free to contact us for more information.
Reference
- Thirumurugan, P.; et al. Click chemistry for drug development and diverse chemical-biology applications. Chemical reviews. 2013, 113(7): 4905-4979.
For research use only. Not intended for any clinical use.
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