High-quality SAM Scaffold Preparation Service via Click Chemistry at CD BioGlyco

Analyzing carbohydrate interactions requires the development of robust and efficient sensors and high-throughput analysis methods. Carbohydrate-based SAM scaffolds have become one of the most promising model systems in this regard. CD BioGlyco provides high-quality SAM scaffold preparation services through click chemistry technology.

• Prepare the metal base: We choose an appropriate metal base, such as gold flakes. Ultrasonic cleaning and treatment are then performed to ensure that the substrate surface is clean and provides reactive sites.
• Preparation of SAM scaffolds: We expose the gold sheet substrate to a solution of click reactants, allowing the click reactants to be adsorbed on the metal surface to form a monomolecular layer. This process is achieved by soaking, spraying, or dropping methods. For example, we immerse the substrate in a toluene solution of 2-propargyl-N-(trimethoxysilylpropyl) ethylcarbamate to form a SAM, The SAM formation of alkyne-terminated silanes is monitored by ellipsometry, contact angle, Fourier transform infrared spectroscopy (FTIR), and X-ray photoelectron spectroscopy (XPS).
• Synthesis of glycosyl click reactants: Under standard Cu(I)-catalyzed conditions, clickable SAMs are easily coupled to azide sugars, including monosaccharides, disaccharides or trisaccharides, uronic acids, etc. We use synthetic chemistry methods to synthesize sugar compounds with click-reactive functional groups. For example, synthesize an azide-terminated glycosylated molecule.
• Click reactions: Copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC) is capable of efficient interphase coupling reactions ("click SAM"), demonstrating its versatility. We dissolve the synthesized glycosylated molecules in an appropriate solvent, and then drop or spray them onto the prepared alkynyl-modified metal substrate to allow click covalent attachment of the carbohydrate compounds to the SAM scaffold. Generally, a copper catalyst is added to promote the click reaction process, and a stable triazole cyclic compound will be formed between the alkynyl group and the azide.
• Washing and processing: After the reaction is completed, we wash and process the sample multiple times to remove unreacted compounds and impurities to ensure high-quality SAM. The use of click chemistry to prepare glycosylated SAM scaffolds provides functional interfaces for a variety of biomedical and biotechnological applications and provides control of specific sugar-receptor interactions, thus having a wide range of roles and potential applications.

Fig.1 GlycoCLICK™-based SAM scaffold preparation service. (CD BioGlyco)

Publication

Applications

• Cell culture: SAM scaffolds are used as cell culture substrates, providing the support required for cell adhesion and growth, and simulating the extracellular matrix environment in organisms.
• Tissue engineering: SAM scaffolds are used to construct three-dimensional structures of artificial tissues and organs, provide support framework and guidance signals for cells, and promote tissue regeneration and repair.
• Interface Materials: SAM scaffolds are used to modify the surface of materials to improve their biocompatibility, anti-pollution, and chemical stability, and are suitable for the application of biomedical devices and materials.

Advantages

• We provide comprehensive solutions from GlycoCLICK™-based SAM scaffold preparation to analysis, providing clients with one-stop service.
• We have reliable quality control systems and stable preparation processes to ensure the consistency and repeatability of the prepared products.
• We use high-quality raw materials and processes to prepare high-level SAM scaffolds in a short time and deliver the prepared products quickly.

CD BioGlyco provides comprehensive and professional GlycoCLICK™-based SAM scaffold preparation service and responds to client needs promptly. Please feel free to contact us if you are interested in our preparation content.

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• GlycoCLICK™-based Hybrid Sugar Material Preparation Service
• GlycoCLICK™-based Polyamidoamine Polycationic Scaffold Preparation Service
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