Description
Biotin-PEG8-amine is a biotinylation reagent featuring a terminal primary amine group. This amine group facilitates the formation of stable amide bonds with carboxyl or phosphate groups. The PEG8 arm enhances the aqueous solubility of the biotin conjugate and reduces steric hindrance during binding to avidin molecules.
Applications
Biotin-PEG8-amine is commonly employed for site-specific biotinylation of biomolecules, enabling their immobilization or detection in various assays. Its versatile amine functionality allows for conjugation to a wide range of molecules, including proteins, peptides, nucleic acids, and surfaces, for applications such as affinity purification, immunohistochemistry, and protein labeling.