Description
Biotin azide plus is a biotinylation reagent designed for labeling glycoproteins and carbohydrate-containing compounds with oxidizable sugars or aldehydes. It comprises a complete copper-chelating system, serving as both reactant and catalyst in CuAAC reactions. The azide functional group enables specific conjugation with alkynes, BCN, or DBCO via- Click Chemistry to form stable triazole linkages.
Applications
Biotin azide plus finds extensive application in bioconjugation experiments, particularly for labeling glycoproteins and carbohydrates. Its versatility allows for precise and efficient biotinylation of biomolecules, enabling downstream applications such as affinity purification, imaging, and proteomic studies. The use of a copper-chelating system ensures robust and reliable labeling under diluted conditions, making it suitable for a wide range of research applications.