Description
Biotin azide is a biotin derivative featuring an azide functional group, enabling it to undergo a copper-catalyzed click reaction with terminal alkynes. This reaction forms a stable triazole linkage, facilitating biotin labeling in various biomolecular applications.
Applications
Biotin azide is widely utilized in bioconjugation and labeling experiments, where it can be specifically coupled to alkyne-modified biomolecules via- click chemistry. This biotinylation method is commonly employed for the detection, purification, and visualization of biomolecules, such as proteins, nucleic acids, and carbohydrates, in various biological assays, including imaging, proteomics, and drug discovery studies.