Azide/Carboxylic acid PEG reagent, N-(azido-PEG4)-biocytin, Purity 98%

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Cat. No.: X24-03-YW0148

Azide/Carboxylic acid PEG reagent, N-(azido-PEG4)-biocytin, Purity 98%

Synonym: 2055042-70-9; N-(azido-PEG4)-Biocytin; AKOS040742214

CAS Number: 2055042-70-9
PubChem CID: 123132114

Size

100 mg; 250 mg; 500 mg

Price

Datasheet

MSDS

Properties

Description

N-(azido-PEG4)-biocytin is a biotin PEG linker featuring a carboxylic group and an azide group. The carboxylic group readily forms stable, irreversible amide bonds with primary amino (-NH₂) groups. The azide group allows for conjugation with alkyne moieties in Cu(I)-catalyzed Click Chemistry reactions or DBCO moieties in copper-free Click Chemistry reactions, forming stable triazole linkages. Additionally, the inclusion of a hydrophilic PEG spacer enhances the solubility of the biotin-conjugated molecules in aqueous media.

Molecular Weight

645.8

Molecular Formula

C₂₇H₄₇N₇O₉S

Functional Group 1

Azide

Functional Group 2

Carboxylic acid

Functional Group 3

None

Reactive Group 1

Alkyne

Reactive Group 2

DBCO

Form

Solid

Purity

98%

Solubility

Water, DMSO, DMF

Identity

Confirmed by NMR.

Applications

N-(azido-PEG4)-biocytin is widely employed in bioconjugation and labeling applications, including protein labeling, cell surface modification, and chemical biology studies. Its versatility allows for precise and efficient biotinylation of biomolecules such as proteins, peptides, and nucleic acids, facilitating various downstream applications such as affinity purification, immunoprecipitation, and fluorescence microscopy.

Storage

Store at -20°C

For Research Use Only. Not For Clinical Use.

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