Description
DNP-PEG12-azide is a heterobifunctional PEG linker comprising a dinitrophenyl group and an azide moiety. Dinitrophenyl serves as a potent FRET quencher, particularly effective when paired with tryptophan or tyrosine residues. Meanwhile, the azide functionality enables click chemistry reactions with terminal alkynes or strained cyclooctynes, facilitating versatile bioconjugation strategies.
Applications
DNP-PEG12-azide can be used in various biophysical and biochemical applications. Its dinitrophenyl group can be employed in fluorescence-based studies, allowing for the investigation of protein-protein interactions or conformational changes. Furthermore, the azide functionality enables efficient conjugation to biomolecules or surfaces via- click chemistry, facilitating the development of labeled probes for imaging, drug delivery systems, or biomaterials engineering. The long PEG12 spacer enhances the compound's solubility and flexibility, ensuring optimal performance in diverse experimental settings.