Biotin PEG reagent, N-(Methyltetrazine-PEG4)-N-biotin-PEG4-acid, Purity 95%

Synonym: BP-28749; N-(Methyltetrazine-PEG4)-N-Biotin-PEG4-acid

PubChem CID: 165430060

Size

25 mg; 50 mg; 100 mg

Price

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Datasheet

Datasheet

MSDS

MSDS

Properties

Description

N-(Methyltetrazine-PEG4)-N-biotin-PEG4-acid is a branched biotinylation reagent featuring a methyltetrazine moiety, a biotin group, and a carboxylic acid functionality. This versatile compound facilitates copper-free click chemistry reactions, particularly with trans-cyclooctene (TCO) reactive reagents. The inverse-electron demand Diels-Alder cycloaddition reaction between TCO and tetrazines is known for its rapid kinetics and outstanding selectivity, making it an ideal tool for bioorthogonal conjugation.

Molecular Weight

923.1

Molecular Formula

C₄₂H₆₆N₈O₁₃S

Functional Group 1

Biotin

Functional Group 2

None

Functional Group 3

None

Form

Reported

Purity

95%

Identity

Confirmed by NMR.

Applications

N-(Methyltetrazine-PEG4)-N-biotin-PEG4-acid is widely employed in bioconjugation and chemical biology studies. Its ability to selectively react with TCO-functionalized molecules via- click chemistry enables efficient labeling of biomolecules without the need for copper catalysts, minimizing non-specific binding and cellular toxicity. This reagent is particularly valuable for the site-specific attachment of biotin tags to proteins, nucleic acids, and other biomolecules of interest, facilitating downstream applications such as affinity purification, protein detection, and cellular imaging.

Storage

Store at -20°C

For Research Use Only. Not For Clinical Use.