Description
N-(Methyltetrazine-PEG4)-N-biotin-PEG4-TFP ester is a multifunctional biotinylation reagent featuring a methyltetrazine moiety, a biotin group, and a TFP (trifluorophenyl) ester functionality. This compound serves as a valuable tool for bioconjugation studies, allowing for copper-free click chemistry reactions with trans-cyclooctene (TCO) reactive reagents. The inverse-electron demand Diels-Alder cycloaddition reaction between TCO and tetrazines is renowned for its rapid kinetics and remarkable selectivity, making it an ideal method for bioorthogonal conjugation. Moreover, the TFP ester moiety provides versatility by enabling conjugation with primary amine groups while exhibiting enhanced stability against hydrolysis compared to traditional NHS esters.
Applications
N-(Methyltetrazine-PEG4)-N-biotin-PEG4-TFP ester can be used in bioconjugation and chemical biology research. Its ability to selectively react with TCO-functionalized molecules via- click chemistry offers a powerful approach for site-specific labeling of biomolecules without the need for copper catalysts, minimizing background signal and cellular toxicity. Furthermore, the TFP ester functionality allows for efficient conjugation with primary amine groups on proteins, peptides, or other biomolecules of interest, providing flexibility in experimental design. This reagent is particularly valuable for applications such as protein labeling, immunofluorescence microscopy, affinity purification, and targeted drug delivery, where precise control over conjugation chemistry and stability is essential for successful outcomes.
