Description
Biotin-PEG4-hydrazide serves as a biotinylation agent suitable for labeling glycoproteins and carbohydrate-containing compounds that possess oxidizable sugars or aldehydes. The hydrazine moiety reacts with aldehydes to create semi-permanent hydrazone bonds. Additionally, the hydrophilic PEG arm enhances water solubility, which is transferred to the biotinylated compound.
Applications
Biotin-PEG4-hydrazide is valuable in bioconjugation and biosensing applications. The biotin moiety allows for specific binding to streptavidin or avidin, enabling the labeling and detection of biomolecules. The PEG4 spacer provides increased solubility and flexibility. It can be used to tag proteins, nucleic acids, or other biomolecules for various assays, such as immunoassays or fluorescence-based detection methods.