Description
N-(Acid-PEG36-PEG3-)-N-bis(PEG3-biotin) is a branched biotin linker designed for protein labeling applications. Featuring a carboxylic acid group, it readily reacts with primary amines in the presence of activators such as EDC and HATU. Additionally, the inclusion of a hydrophilic PEG branch enhances the water solubility of the compound, facilitating its use in aqueous environments.
Applications
N-(Acid-PEG36-PEG3-)-N-bis(PEG3-biotin) can be used in protein labeling and immobilization strategies. The carboxylic acid functionality enables efficient conjugation with primary amines on proteins, forming stable amide bonds. The resulting biotinylated proteins can then be used for various downstream applications, including affinity purification, protein detection, and immunoprecipitation assays, utilizing the strong and specific interaction between biotin and avidin/streptavidin.