Biotin/Maleimide PEG reagent, Biotin-PEG3-Mal, Purity 98%

Synonym: 1431618-70-0; Biotin-PEG3-Mal; Biotin-PEG3-NH-Mal; (+)-biotin-PEG3-NH-Mal; N-(15-(2,5-Dioxo-2,5-dihydro-1H-pyrrol-1-yl)-13-oxo-3,6,9-trioxa-12-azapentadecyl)-5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide

CAS Number: 1431618-70-0
PubChem CID: 77078216

Size

100 mg; 250 mg; 1 g

Price

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Datasheet

Datasheet

MSDS

MSDS

Properties

Description

Biotin-PEG3-Mal is a maleimide-based biotinylation reagent designed to selectively react with sulfhydryl groups. This compound forms a stable thioether linkage when coupled to sulfhydryl-containing biomolecules under mildly acidic conditions (pH 6.5-7.5).

Molecular Weight

569.7

Molecular Formula

C₂₅H₃₉N₅O₈S

Functional Group 1

Biotin

Functional Group 2

Maleimide

Functional Group 3

None

Reactive Group 1

Sulfhydryl

Form

Solid

Purity

98%

Solubility

DMSO, DCM, DMF

Identity

Confirmed by NMR.

Applications

Biotin-PEG3-Mal finds widespread application in the site-specific biotinylation of proteins, peptides, antibodies, and other thiol-containing biomolecules. The resulting biotinylated conjugates are commonly used in various biological assays, such as pull-down assays, enzyme-linked immunosorbent assays (ELISA), and fluorescence-based imaging techniques. Additionally, the versatility of this reagent allows for the selective modification of cysteine residues in proteins for functional studies or protein labeling purposes.

Storage

Store at -20°C

For Research Use Only. Not For Clinical Use.