Biotin/Maleimide PEG reagent, Biotin-PEG6-Mal, Purity 98%

Synonym: 1808990-66-0; Biotin-PEG6-Mal; Biotin-PEG6-maleimide; Biotin-PEG6-NH-Mal; (+)-biotin-PEG6-NH-Mal

CAS Number: 1808990-66-0
PubChem CID: 77078215

Size

100 mg; 250 mg; 500 mg

Price

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Datasheet

Datasheet

MSDS

MSDS

Properties

Description

Biotin-PEG6-Mal is a maleimide biotinylation linker designed to react specifically with sulfhydryl groups (at pH 6.5-7.5) to form stable thioether linkages. The inclusion of a hydrophilic PEG spacer enhances the aqueous solubility of molecules conjugated to the biotin compound while reducing steric hindrance during binding to avidin molecules.

Molecular Weight

701.8

Molecular Formula

C₃₁H₅₁N₅O₁₁S

Functional Group 1

Biotin

Functional Group 2

Maleimide

Functional Group 3

None

Reactive Group 1

Sulfhydryl

Form

Solid (low melting point)

Purity

98%

Solubility

DCM

Identity

Confirmed by NMR.

Applications

Biotin-PEG6-Mal finds widespread application in the site-specific labeling of sulfhydryl-containing biomolecules such as proteins, peptides, and antibodies. The conjugation of biotin enables subsequent detection, purification, or immobilization of the labeled biomolecules using streptavidin-based assays, including Western blotting, ELISA, and immunoprecipitation.

Storage

Store at -20°C

For Research Use Only. Not For Clinical Use.