Cat. No.: X24-03-YW0147
Biotin/NHS PEG reagent, Biotin-EDA-PEG5-NHS, Purity 98%
Synonym: BP-21744; (2,5-Dioxopyrrolidin-1-yl) 3-[2-[2-[2-[2-[3-[2-[5-[(3aS,4S,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoylamino]ethylamino]-3-oxopropoxy]ethoxy]ethoxy]ethoxy]ethoxy]propanoate
Properties
Description
Biotin-EDA-PEG5-NHS is a biotinylation reagent featuring an N-hydroxysuccinimide (NHS) ester functional group, designed for straightforward and efficient biotinylation of antibodies, proteins, and other primary amine-containing biomolecules. The NHS-activated biotin compound readily reacts with primary amino groups (-NH2) to establish stable and irreversible amide bonds. Additionally, the inclusion of a hydrophilic PEG spacer arm enhances the water solubility of the biotinylated molecule.
Molecular Formula
C30H49N5O12S
Functional Group 1
Biotin
Identity
Confirmed by NMR.
Applications
Biotin-EDA-PEG5-NHS finds extensive utility in various biochemical and molecular biology applications, including affinity purification, immunoprecipitation, protein labeling, and detection assays such as ELISA and Western blotting. Its versatility allows for precise and site-specific biotinylation of target molecules, enabling downstream applications such as protein purification, cellular imaging, and detection of protein-protein interactions. The hydrophilic PEG spacer arm ensures optimal solubility and compatibility with aqueous environments, facilitating efficient and reliable labeling processes.
For Research Use Only. Not For
Clinical Use.
