Biotin/NHS PEG reagent, Biotin-PEG3-NHS ester, Purity 98%

Synonym: 1253286-56-4; Biotin-PEG3-NHS ester; BIOTIN-PEG3-NHSESTER; 2,5-Dioxopyrrolidin-1-yl 14-oxo-18-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)-4,7,10-trioxa-13-azaoctadecanoate; Biotin-PEG3-NHS

CAS Number: 1253286-56-4
PubChem CID: 77078478

Size

250 mg; 500 mg; 1 g

Price

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Datasheet

Datasheet

MSDS

MSDS

Properties

Description

Biotin-PEG3-NHS ester is a biotinylation compound designed to couple with primary amino groups (-NH₂). The NHS group reacts specifically and efficiently with lysine and N-terminal amino groups at pH 7-9 to form stable amide bonds.

Molecular Weight

544.6

Molecular Formula

C₂₃H₃₆N₄O₉S

Functional Group 1

Biotin

Functional Group 2

NHS

Functional Group 3

None

Reactive Group 1

Amine

Form

Solid

Purity

98%

Identity

Confirmed by NMR.

Applications

Biotin-PEG3-NHS ester is commonly used for the biotinylation of proteins, peptides, antibodies, and other biomolecules. This reaction enables the attachment of biotin tags to target molecules for various applications such as protein purification, detection, and immobilization in assays like immunoprecipitation, Western blotting, and enzyme-linked immunosorbent assays (ELISA).

Storage

Store at -20°C

For Research Use Only. Not For Clinical Use.