Description
Biotin-PEG6-NHS ester is an amine-reactive biotinylation reagent containing a PEG spacer. The NHS ester moiety facilitates efficient coupling with amino (-NH2) containing molecules, forming stable amide bonds under mild conditions.
Applications
Biotin-PEG6-NHS ester is widely used for the site-specific biotinylation of proteins, peptides, antibodies, and other biomolecules. It enables the introduction of biotin tags for various applications, including affinity purification, protein detection, protein-protein interaction studies, and imaging experiments. The PEG spacer enhances water solubility and reduces steric hindrance, ensuring efficient labeling and maintaining the native structure and function of the biomolecules.