Cat. No.: X24-03-YW0123
Biotin/NHS PEG reagent, NHS-PEG4-biotinidase resistant biotin, Purity 95%
Synonym: 1334172-61-0; 18-Biotinamino-17-oxo-4,7,10,13-tetraoxa-16-azaicosan-1-oic acid succinimidyl ester; NHS-PEG4-biotinidase resistant biotin; (2,5-Dioxopyrrolidin-1-yl) 3-[2-[2-[2-[2-[2-[5-[(3aS,4S,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoylamino]butanoylamino]ethoxy]ethoxy]ethoxy]ethoxy]propanoate; DTXSID101099287
- CAS Number: 1334172-61-0
- PubChem CID: 121234017
Properties
Description
NHS-PEG4-biotinidase resistant biotin is a versatile biotinylation reagent featuring a terminal NHS ester for conjugation purposes. The inclusion of a hydrophilic PEG spacer enhances solubility in aqueous environments. The terminal biotin moiety can be selectively coupled to amine molecules using activators such as EDC or HATU. Additionally, the Fmoc group can be readily deprotected under basic conditions, yielding a free amine suitable for further conjugation reactions.
Molecular Formula
C29H47N5O11S
Functional Group 1
Biotin
Identity
Confirmed by NMR.
Applications
NHS-PEG4-biotinidase resistant biotin can be used in protein labeling, oligonucleotide modification, and bioconjugation studies. The NHS ester functionality enables specific labeling of primary amines (-NH2) present in proteins, peptides, antibodies, and other biomolecules. The hydrophilic PEG spacer ensures optimal solubility and stability in aqueous media, facilitating efficient coupling reactions. Moreover, the presence of the biotin moiety imparts biotinidase resistance, allowing for robust and long-lasting biotinylation in biological systems.
For Research Use Only. Not For
Clinical Use.
