Description
Dde biotin-PEG4-picolyl azide is a cleavable biotin reagent featuring a picolyl azide moiety, facilitating conjugation with alkyne-containing biomolecules. This biotin reagent includes a copper-chelating motif that significantly enhances the Cu(I)-catalyzed Click Chemistry reaction. The hydrophilic spacer arm ensures improved solubility of the labeled molecules in aqueous media.
Applications
Dde biotin-PEG4-picolyl azide is extensively utilized in bioconjugation studies for labeling biomolecules, including proteins, peptides, and nucleic acids. The specific attachment of biotin to target molecules enables subsequent affinity purification, detection, and visualization using streptavidin-based assays such as Western blotting, immunofluorescence, and flow cytometry.